Palladium(II)-Mediated Assembly of Biotinylated Ion Channels

Craig P. Wilson, Cecile Boglio, Long Ma, Scott L. Cockroft, Simon J. Webb

Research output: Contribution to journalArticlepeer-review

Abstract

Simple synthetic methodology has been used to create biotinylated pyridyl cholate lipids that can undergo multiple self-assembly events when inserted into phospholipid vesicles; Pd-II links cholates into transmembrane lipids, while avidin laterally clusters these complexes together and concomitantly assembles the vesicles into aggregates. The transmembrane assembly of cholates by Pd-II "opened" the ion channels, whereas avidin addition produced vesicle aggregates, giving a system that mimicked both transmembrane transport and cellular adhesion. Complexation of these Pd-II-linked cholates by avidin gave a measurable decrease in ion flow, suggesting some channels became blocked or were prevented from adopting the optimum geometry for ion conduction. This reflects the importance of spatially appropriate preorganisation when generating active supramolecular assemblies.

Original languageEnglish
Pages (from-to)3465-3473
Number of pages9
JournalChemistry - A European Journal
Volume17
Issue number12
DOIs
Publication statusPublished - Mar 2011

Keywords

  • ion channels
  • membranes
  • molecular recognition
  • self assembly
  • vesicles

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