Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus

Michael J Devine; Mina Ryten; Petr Vodicka; Alison J Thomson; Tom Burdon; Henry Houlden; Fatima Cavaleri; Masumi Nagano; Nicola J Drummond; Jan-Willem Taanman; Anthony H Schapira; Katrina Gwinn; John Hardy; Patrick A Lewis; Tilo Kunath

doi:10.1038/ncomms1453

Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus

Michael J Devine, Mina Ryten, Petr Vodicka, Alison J Thomson, Tom Burdon, Henry Houlden, Fatima Cavaleri, Masumi Nagano, Nicola J Drummond, Jan-Willem Taanman, Anthony H Schapira, Katrina Gwinn, John Hardy, Patrick A Lewis, Tilo Kunath

Research output: Contribution to journal › Article › peer-review

Abstract

A major barrier to research on Parkinson's disease is inaccessibility of diseased tissue for study. One solution is to derive induced pluripotent stem cells from patients and differentiate them into neurons affected by disease. Triplication of SNCA, encoding α-synuclein, causes a fully penetrant, aggressive form of Parkinson's disease with dementia. α-Synuclein dysfunction is the critical pathogenic event in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. Here we produce multiple induced pluripotent stem cell lines from an SNCA triplication patient and an unaffected first-degree relative. When these cells are differentiated into midbrain dopaminergic neurons, those from the patient produce double the amount of α-synuclein protein as neurons from the unaffected relative, precisely recapitulating the cause of Parkinson's disease in these individuals. This model represents a new experimental system to identify compounds that reduce levels of α-synuclein, and to investigate the mechanistic basis of neurodegeneration caused by α-synuclein dysfunction.

Original language	English
Article number	440
Number of pages	10
Journal	Nature Communications
Volume	2
Issue number	n/a
DOIs	https://doi.org/10.1038/ncomms1453
Publication status	Published - Aug 2011

Keywords / Materials (for Non-textual outputs)

Cell Differentiation
Cells, Cultured
Gene Dosage
Humans
Induced Pluripotent Stem Cells
Neurons
Parkinson Disease
alpha-Synuclein

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Devine, M. J., Ryten, M., Vodicka, P., Thomson, A. J., Burdon, T., Houlden, H., Cavaleri, F., Nagano, M., Drummond, N. J., Taanman, J.-W., Schapira, A. H., Gwinn, K., Hardy, J., Lewis, P. A., & Kunath, T. (2011). Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus. Nature Communications, 2(n/a), Article 440. https://doi.org/10.1038/ncomms1453

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abstract = "A major barrier to research on Parkinson's disease is inaccessibility of diseased tissue for study. One solution is to derive induced pluripotent stem cells from patients and differentiate them into neurons affected by disease. Triplication of SNCA, encoding α-synuclein, causes a fully penetrant, aggressive form of Parkinson's disease with dementia. α-Synuclein dysfunction is the critical pathogenic event in Parkinson's disease, multiple system atrophy and dementia with Lewy bodies. Here we produce multiple induced pluripotent stem cell lines from an SNCA triplication patient and an unaffected first-degree relative. When these cells are differentiated into midbrain dopaminergic neurons, those from the patient produce double the amount of α-synuclein protein as neurons from the unaffected relative, precisely recapitulating the cause of Parkinson's disease in these individuals. This model represents a new experimental system to identify compounds that reduce levels of α-synuclein, and to investigate the mechanistic basis of neurodegeneration caused by α-synuclein dysfunction.",

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Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

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Control of development and reproductive traits

Cite this

Parkinson's disease induced pluripotent stem cells with triplication of the α-synuclein locus

Abstract

Keywords / Materials (for Non-textual outputs)

Access to Document

Other files and links

Fingerprint

Projects

Control of development and reproductive traits

Cite this