Abstract / Description of output
OBJECTIVE: To determine the prevalence of human parvovirus B19 infection in patients with juvenile idiopathic arthritis (JIA) by detection of specific IgM, IgG, and viral DNA.
METHODS: Serum samples of 50 patients with diagnosis of JIA and 39 healthy controls were analyzed by ELISA to detect IgG and IgM anti-B19-specific antibodies. The parvovirus B19 genome was detected by nested polymerase chain reaction (PCR). The average age of the patients was 9.6 years (2-14 yrs); 30 were female (60%) and 20 male (40%). The definitive diagnoses of these patients corresponded to 19 systemic forms (38%), 11 to the oligoarticular variety (22%) and 20 to the polyarticular (40%). The average age of the control group was 7.8 years (2-16 yrs); the distribution by sex was 25 females (64%) and 14 males (36%).
RESULTS: IgM against parvovirus B19 was detected in 20% of the cases (10 patients) and B19 DNA genome by PCR in 48% (24 patients); in 10% of the cases (5 patients), both markers were detected. IgG was found in 32% (16 patients). In the control group neither IgM nor the viral genome was detected. However, 43.5% of the controls (17/39) had IgG against parvovirus B19, indicating past infection by the virus.
CONCLUSION: Our study confirms recent observations regarding a high prevalence of viral DNA in JIA patients and a possible role of this viral infection in JIA pathogenesis.
Original language | English |
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Pages (from-to) | 1336-40 |
Number of pages | 5 |
Journal | The Journal of rheumatology |
Volume | 34 |
Issue number | 6 |
Publication status | Published - Jun 2007 |
Keywords / Materials (for Non-textual outputs)
- Adolescent
- Antibodies, Viral
- Arthritis, Juvenile
- Case-Control Studies
- Child
- Child, Preschool
- DNA, Viral
- Female
- Humans
- Immunoglobulin G
- Immunoglobulin M
- Infant
- Male
- Parvoviridae Infections
- Parvovirus B19, Human
- Prevalence