Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis

Jayne Culley, Peter W Nagle, John C Dawson, Neil O Carragher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Three dimensional models of cell culture enables researchers to recreate aspects of tumour biology not replicated by traditional two dimensional techniques. Here we describe a protocol to enable automated high throughput phenotypic profiling across panels of patient derived glioma stem cell spheroid models. We demonstrate the use of both live/dead cell end-points and monitor the dynamic changes in the cell cycle using cell lines expressing the FUCCI cell cycle reporter. Together, these assays provide additional insight into the mechanism of action of compound treatments over traditional cell viability assay endpoints.
Original languageEnglish
JournalSlas Discovery
Early online date15 Sep 2022
DOIs
Publication statusE-pub ahead of print - 15 Sep 2022

Keywords

  • Glioblastoma
  • 3-dimensional
  • Spheroid
  • Cell Cycle
  • Drug Discovery

Fingerprint

Dive into the research topics of 'Patient derived glioma stem cell spheroid reporter assays for live cell high content analysis'. Together they form a unique fingerprint.

Cite this