TY - JOUR
T1 - PCR screening of mammalian predators (Carnivora) for adenoviruses and herpesviruses
T2 - The first detection of a mustelid herpesvirus in Hungary
AU - Eszter, Dandár
AU - László, Szabó
AU - Miklós, Heltai
AU - Doszpoly, Andor
PY - 2010
Y1 - 2010
N2 - The purpose of this study was to conduct a survey on the occurrence of adenoviruses and herpesviruses in different mammalian predators in Hungary. To this end, samples were collected from 75 individuals including native free living and zoo animals. For the presence of adenovirus, only two samples of golden jackals (Canis aureus) were found to be positive. According to the sequence analysis of the PCR products, both samples contained a virus identical with canine adenovirus type 2 (CAdV-2). For herpesviruses, merely one sample was positive. This was the single sample originating from a European badger (Afe/es meles). The nucleotide sequence of the PCR product was almost identical with the corresponding sequence of the Mustelid herpesvirus 1 (MuHV-1) that has been recently isolated in Great Britain. To increase the sample number, the carnivorous source was the only selection criterion during the collection, and excretes of apparently healthy zoo animals as well as internal organs from animals shot during hunting were also included. Thus, the majority of the examined samples were from clinically healthy individuals. In the case of both viruses, a very sensitive, nested PCR method was used. The chances to detect these viruses, which are prone to establish persistent infection, are probably limited to animals that are in active viraemic status. One major finding is the first demonstration of the presence of MuHV-1 in Hungary and also in the European continent. Moreover, this is the first report on the molecular detection of CAdV-2 in European golden jackals.
AB - The purpose of this study was to conduct a survey on the occurrence of adenoviruses and herpesviruses in different mammalian predators in Hungary. To this end, samples were collected from 75 individuals including native free living and zoo animals. For the presence of adenovirus, only two samples of golden jackals (Canis aureus) were found to be positive. According to the sequence analysis of the PCR products, both samples contained a virus identical with canine adenovirus type 2 (CAdV-2). For herpesviruses, merely one sample was positive. This was the single sample originating from a European badger (Afe/es meles). The nucleotide sequence of the PCR product was almost identical with the corresponding sequence of the Mustelid herpesvirus 1 (MuHV-1) that has been recently isolated in Great Britain. To increase the sample number, the carnivorous source was the only selection criterion during the collection, and excretes of apparently healthy zoo animals as well as internal organs from animals shot during hunting were also included. Thus, the majority of the examined samples were from clinically healthy individuals. In the case of both viruses, a very sensitive, nested PCR method was used. The chances to detect these viruses, which are prone to establish persistent infection, are probably limited to animals that are in active viraemic status. One major finding is the first demonstration of the presence of MuHV-1 in Hungary and also in the European continent. Moreover, this is the first report on the molecular detection of CAdV-2 in European golden jackals.
UR - http://www.scopus.com/inward/record.url?scp=77952817789&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:77952817789
SN - 0025-004X
VL - 132
SP - 302
EP - 308
JO - Magyar Allatorvosok Lapja
JF - Magyar Allatorvosok Lapja
IS - 5
ER -