PDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny

Diana Sá da Bandeira, Alastair Morris Kilpatrick, Madalena Marques, Mario Gomez-Salazar, Telma Ventura, Zaniah Nashira Gonzalez , Dorota Stefancova, Fiona Rossi, Matthieu Vermeren, Chris Sebastiaan Vink, Mariana Beltran, Neil Cowan Henderson, Bongnam Jung, Reinier Van Der Linden, Harmen Jan George van de Werken, Wilfred F J van IJcken, Christer Betsholtz, Stuart John Forbes, Henar Cuervo, Mihaela Crisan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires
HSC specification signals from the surrounding microenvironment. In zebrafish, PDGFB/
PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is
required in the HSC specification niche. Little is known about murine HSPC specification in
vivo and whether PDGF-B/PDGFRβ is involved. Here we show that PDGFRβ is expressed in
distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its
deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine
hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+
embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings
provide crucial information for the controlled production of HSPCs in vitro.
Original languageEnglish
JournalCell Reports
Issue number3
Publication statusPublished - 19 Jul 2022


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