Perinuclear antineutrophil cytoplasmic autoantibodies in dogs infected with various vector-borne pathogens and in dogs with immune-mediated hemolytic anemia.

Anna Karagianni, Laia Solano-Gallego, E.b. Breitschwerdt, FP Gaschen, M. J. Day, B Wieland, Karin Allenspach

Research output: Contribution to journalArticlepeer-review

Abstract

Objective—To determine the prevalence of perinuclear antineutrophil cytoplasmic autoantibodies
(pANCA) in dogs with confirmed or suspected immune-mediated hemolytic
anemia (IMHA) or dogs infected with various vector-borne pathogens, including Rickettsia
rickettsii, Bartonella henselae, Bartonella vinsonii subsp berkhoffii, Ehrlichia canis, Borrelia
burgdorferi, and Leishmania infantum.
Animals—55 dogs with confirmed or suspected IMHA, 140 dogs seroreactive for vectorborne
pathogens, and 62 healthy dogs and dogs seronegative for vector-borne pathogens.
Procedures—Samples were allocated to subgroups on the basis of the health status of
the dogs and the degree of seroreactivity against various vector-borne pathogens. Serum
samples were tested retrospectively via indirect immunofluorescence assay to determine
pANCA status.
Results—26 of 55 (47%) dogs with confirmed or suspected IMHA and 67 of 140 (48%) dogs
seroreactive for vector-borne pathogens had positive results when tested for pANCA. Serum
samples with the highest antibody concentrations against L infantum antigen had the highest
proportion (28/43 [65%]) that were positive for pANCA. One of 20 (5%) dogs seronegative for
tick-borne pathogens and 8 of 22 (36%) dogs seronegative for L infantum had positive results
for pANCA. One of 20 (5%) healthy dogs had serum antibodies against pANCA.
Conclusions and Clinical Relevance—pANCA were detected in a high percentage of dogs
with IMHA and vector-borne infectious diseases. Therefore, pANCA may be a relatively nonspecific
marker for dogs with inflammatory bowel disease, although they could represent
a biomarker for immune-mediated diseases and infections. (Am J Vet Res 2012;73:1403–
1409)
colitis.1,7,8 Detection via IFA of pANCA on eth-

Original languageEnglish
JournalAmerican Journal of Veterinary Research
DOIs
Publication statusPublished - 2012

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