Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

Vicky J. Young, Syed F. Ahmad, Jeremy K. Brown, W. Colin Duncan, Andrew W. Horne*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n=16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n=13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations (P

Original languageEnglish
Article number16859
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 18 Nov 2015

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