Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

Vicky J. Young; Syed F. Ahmad; Jeremy K. Brown; W. Colin Duncan; Andrew W. Horne

doi:10.1038/srep16859

Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

Vicky J. Young, Syed F. Ahmad, Jeremy K. Brown, W. Colin Duncan, Andrew W. Horne^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n=16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n=13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations (P

Original language	English
Article number	16859
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep16859
Publication status	Published - 18 Nov 2015

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title = "Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis",

abstract = "VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n=16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n=13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations (P",

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AU - Young, Vicky J.

AU - Ahmad, Syed F.

AU - Brown, Jeremy K.

AU - Duncan, W. Colin

AU - Horne, Andrew W.

PY - 2015/11/18

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N2 - VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n=16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n=13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations (P

AB - VEGF-A, an angiogenic factor, is increased in the peritoneal fluid of women with endometriosis. The cytokine TGF-β1 is thought to play a role in the establishment of endometriosis lesions. Inhibitor of DNA binding (ID) proteins are transcriptional targets of TGF-β1 and ID1 has been implicated in VEGF-A regulation during tumor angiogenesis. Herein, we determined whether peritoneal expression of VEGF-A is regulated by TGF-β1 through the ID1 pathway in women with endometriosis. VEGF-A was measured in peritoneal fluid by ELISA (n=16). VEGF-A and ID1 expression was examined in peritoneal biopsies (n=13), and primary peritoneal and immortalized mesothelial cells (MeT5A) by immunohistochemistry, qRT-PCR and ELISA. VEGF-A was increased in peritoneal fluid from women with endometriosis and levels correlated with TGF-β1 concentrations (P

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Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

Abstract

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Toward new treatments for endometriosis: is the peritoneal transforming growth factor: B superfamily a therapeutic target?

Centre for Reproductive Resillience

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Peritoneal VEGF-A expression is regulated by TGF-β1 through an ID1 pathway in women with endometriosis

Abstract

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Toward new treatments for endometriosis: is the peritoneal transforming growth factor: B superfamily a therapeutic target?

Centre for Reproductive Resillience

Cite this