Abstract
Objective
To investigate the impact of intermittent interleukin-2 (IL-2) plus combination antiretroviral therapy (cART) on HIV-1 entry co-receptor use.
Methods
Primary HIV-1 isolates were obtained from 54 HIV-1-positive individuals at baseline and after 12 months using co-cultivation of peripheral blood mononuclear cells (PBMC) with activated PBMC of HIV-negative healthy donors. HIV-1 co-receptor use was determined on U87-CD4 cells.
Results
Fourteen out of the 21 (67%) IL-2-treated individuals harbouring a primary CCR5-dependent (R5) HIV-1 isolate at baseline confirmed an R5 virus isolation after 12 months in contrast to 3 out of 7 (43%) of those receiving cART only. After 12 months, only 1 R5X4 HIV-1 isolate was obtained from 21 cART+IL-2-treated individuals infected with an R5 virus at entry (5%) vs. 2/7 (29%) patients receiving cART alone, as confirmed by a 5-year follow-up on some individuals.
Conclusions
Intermittent IL-2 administration plus cART may prevent evolution towards CXCR4 usage in individuals infected with R5 HIV-1.
Original language | English |
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Pages (from-to) | 349-352 |
Number of pages | 4 |
Journal | HIV Medicine |
Volume | 11 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2010 |
Keywords / Materials (for Non-textual outputs)
- cART
- CCR5
- CXCR4
- HIV isolation
- IL-2 therapy
- viremia
- CHEMOKINE RECEPTOR EXPRESSION
- SYNCYTIUM-INDUCING PHENOTYPE
- HIV-INFECTED PATIENTS
- T-LYMPHOCYTES
- DISEASE PROGRESSION
- PROGNOSTIC VALUE
- THERAPY
- CORECEPTOR
- IL-2
- AIDS