Perturbations in nitric oxide homeostasis promote Arabidopsis disease susceptibility towards Phytophthora parasitica

Beimi Cui, Xiangren Ma, Yuan Li, Yu Zhou, Xiuyun Ju, Adil Hussain, Saima Umbreen, Bo Yuan, Anika Tabassum, Jibril Lubega, Weixing Shan, Gary J. Loake, Qiaona Pan

Research output: Contribution to journalArticlepeer-review

Abstract

Phytophthora species can infect hundreds of different plants including many important crops causing a number of agriculturally relevant diseases. A key feature of attempted pathogen infection is the rapid production of the redox active molecule, nitric oxide (NO). However, the potential role(s) of NO in plant resistance against Phytophthora is relatively unexplored. Here we show that the level of NO accumulation is crucial for basal resistance in Arabidopsis against Phytophthora parasitica. Counterintuitively, both relatively low or relatively high NO accumulation leads to reduced resistance against P. parasitica. S-nitrosylation, the addition of a NO group to a protein cysteine thiol to form an S-nitrosothiol (SNO), is an important route for NO bioactivity and this process is regulated predominantly by S-nitrosoglutathione reductase 1 (GSNOR1). Loss-of-function mutations in GSNOR1 disable both salicylic acid (SA) accumulation and associated signalling and also the production of reactive oxygen species (ROS), leading to susceptibility towards P. parasitica. Significantly, we also demonstrate that secreted proteins from P. parasitica can inhibit Arabidopsis GSNOR1 activity.
Original languageEnglish
JournalMolecular Plant Pathology
Publication statusAccepted/In press - 5 Jun 2021

Keywords

  • nitric oxide
  • phytophthora parasitica
  • reactive oxygen species
  • salicylic acid
  • s-nitrosylation

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