Projects per year
Abstract
Assessing the retention of cell therapies following implantation is vital and often achieved by labelling cells with 2′-[18F]-fluoro-2′-deoxy-D-glucose (18F-FDG). However, this approach is limited by local retention of cell-effluxed radiotracer. Here, in a preclinical model of critical limb ischemia, we assessed a novel method of cell tracking using 3′-deoxy-3′-L-[18F]-fluorothymidine (18F-FLT); a clinically available radiotracer which we hypothesise will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention. Human endothelial cells (HUVECs) were incubated with 18F-FDG or 18F-FLT and cell characteristics were evaluated. Dynamic positron emission tomography (PET) images were acquired post-injection of free 18F-FDG/18F-FLT or 18F-FDG/18F-FLT-labelled HUVECs, following the surgical induction of mouse hind-limb ischemia. In vitro, radiotracer incorporation and efflux was similar with no effect on cell viability, function or proliferation under optimised conditions (5 MBq/mL, 60 min). Injection of free radiotracer demonstrated a faster clearance of 18F-FLT from the injection site vs. 18F-FDG (p ≤ 0.001), indicating local cellular uptake. Using 18F-FLT-labelling, estimation of HUVEC retention within the engraftment site 4 hr post-administration was 24.5 ± 3.2%. PET cell tracking using 18F-FLT labelling is an improved approach vs. 18F-FDG as it is not susceptible to local host cell reuptake, resulting in a more accurate estimation of cell retention.
Original language | English |
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Article number | 44233 |
Journal | Scientific Reports |
DOIs | |
Publication status | Published - 13 Mar 2017 |
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Dive into the research topics of 'PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer'. Together they form a unique fingerprint.Projects
- 4 Finished
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CH/09/002/26360 BHF Scholarship Scheme for Dr Russell Everett
Newby, D. (Principal Investigator)
1/08/15 → 31/10/16
Project: Research
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Identification and prediction of coronary artery plaque rupture using 18F-fluoride positron emission tomography
Newby, D. (Principal Investigator)
1/01/15 → 30/06/22
Project: Research
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BHF Research Excellence Award (2) (renewal) Grant 1
Mullins, J. (Principal Investigator), Bailey, M. (Co-investigator), Beqqali, A. (Co-investigator), Caporali, A. (Co-investigator), Crisan, M. (Co-investigator), Mansley, M. (Co-investigator), Meloni, M. (Co-investigator), Reynolds, R. (Co-investigator) & Wu, J. (Co-investigator)
1/04/14 → 30/06/19
Project: Research
Profiles
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Adriana Tavares
- Deanery of Clinical Sciences - Personal Chair of Translational Molecular Imaging
- Centre for Cardiovascular Science
- Edinburgh Imaging
Person: Academic: Research Active