Phenotypic and genotypic alterations associated with the attenuation of a Theileria annulata vaccine cell line from Turkey

RPT Somerville, P Littlebury, E Pipano, CGD Brown, [No Value] Shkap, RE Adamson, RA Oliver, EJ Glass, FR Hall*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Attenuated vaccines, produced by prolonged in vitro culture of the macroschizont stage of the life-cycle, are the main method of controlling Theileria annulata infections. Little is known about the mechanism(s) of attenuation. Here we present data from a Turkish cell line demonstrating that attenuation is associated with reduced ability to differentiate into microschizonts and a reduction in matrix metalloproteinase activity. We also show that attenuation results in a change in the structure of the parasite population. Using the technique of differential mRNA display, we demonstrate that gene expression profiles differ between non-attenuated and attenuated macroschizont infected leucocytes. One differentially expressed gene is of parasite origin. These data are discussed in the context of a multifactorial model of virulence. (C) 1998 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)569-575
Number of pages7
JournalVaccine
Volume16
Issue number6
Publication statusPublished - Apr 1998

Keywords

  • Theileria annulata
  • attenuation
  • vaccine
  • BABESIA-BOVIS
  • SURFACE-ANTIGEN
  • SUBPOPULATIONS
  • POLYMORPHISM
  • EXPRESSION
  • MEROZOITE
  • SELECTION
  • CATTLE
  • DNA

Fingerprint

Dive into the research topics of 'Phenotypic and genotypic alterations associated with the attenuation of a Theileria annulata vaccine cell line from Turkey'. Together they form a unique fingerprint.

Cite this