Photocontrol of subcellular partitioning of phytochrome-B:GFP fusion protein in tobacco seedlings

P Gil, S Kircher, E Adam, E Bury, L Kozma-Bognar, E Schäfer, F Nagy

Research output: Contribution to journalArticlepeer-review

Abstract

Photomorphogenesis of higher plants is regulated by photoreceptors including the red/far-red light-absorbing phytochromes, blue-UV/A sensing cryptochromes and as yet uncharacterized UV/B receptors. Specific phototransduction pathways that are controlled by either individual or interacting photoreceptors mediate regulation. Phytochrome B (phyB) is the major red light-sensing photoreceptor. Phototransduction mediated by this light sensor has been shown to include light-dependent nuclear import and interaction of phyB with transcription factor-like proteins in the nucleus. Here we report that nuclear import of phyB and physiological responses regulated by this photoreceptor exhibit very similar wavelength- and fluence rate-dependence. Nuclear import of phyB is insensitive to single red, blue and far-red light pulses. It is induced by continuous red light and to a lesser extent by continuous blue light, whereas far-red light is completely ineffective. The data presented indicate that light-dependent partitioning of phyB exhibits features characteristic of blue light responsiveness amplification, a phenomenon that is thought to be mediated by interaction of phyB with CRY1.
Original languageEnglish
Pages (from-to)135-45
Number of pages11
JournalThe Plant Journal
Volume22
Issue number2
Publication statusPublished - 2000

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