Pif1-Family Helicases Support Fork Convergence during DNA Replication Termination in Eukaryotes

Tom D Deegan, Jonathan Baxter, María Ángeles Ortiz Bazán, Joseph T P Yeeles, Karim P M Labib

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The convergence of two DNA replication forks creates unique problems during DNA replication termination. In E. coli and SV40, the release of torsional strain by type II topoisomerases is critical for converging replisomes to complete DNA synthesis, but the pathways that mediate fork convergence in eukaryotes are unknown. We studied the convergence of reconstituted yeast replication forks that include all core replisome components and both type I and type II topoisomerases. We found that most converging forks stall at a very late stage, indicating a role for additional factors. We showed that the Pif1 and Rrm3 DNA helicases promote efficient fork convergence and completion of DNA synthesis, even in the absence of type II topoisomerase. Furthermore, Rrm3 and Pif1 are also important for termination of plasmid DNA replication in vivo. These findings identify a eukaryotic pathway for DNA replication termination that is distinct from previously characterized prokaryotic mechanisms.

Original languageEnglish
Pages (from-to)231-244.e9
JournalMolecular Cell
Issue number2
Publication statusPublished - 18 Apr 2019

Keywords / Materials (for Non-textual outputs)

  • DNA Helicases/genetics
  • DNA Replication/genetics
  • DNA Topoisomerases/genetics
  • Escherichia coli/genetics
  • Eukaryota/genetics
  • Genomic Instability
  • Plasmids/genetics
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins/genetics


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