Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Anton Puzorjov; Mert Ünal; Martin A  Wear; Alistair J McCormick

doi:10.1016/j.biortech.2021.126459

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Anton Puzorjov, Mert Ünal, Martin A Wear, Alistair J McCormick

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is presented. Batch cultivation without antibiotics had no impact on growth or Te-PC production and optimisation of growth conditions resulted in Te-PC contents of 75.3 ± 1.7 mg g DW-1. Wet biomass was harvested following chitosan-based flocculation with a 95 ± 1% efficiency, and Te-PC was extracted by high pressure homogenisation. Subsequent purification by heat-treatment and two-step ammonium sulfate precipitation removed chlorophyll and allophycocyanin contamination, resulting in Te-PC purities of 2.9 ± 0.7 and a mean Te-PC recovery of 84 ± 12%.

Original language	English
Article number	126459
Number of pages	10
Journal	Bioresource technology
Volume	345
Early online date	1 Dec 2021
DOIs	https://doi.org/10.1016/j.biortech.2021.126459
Publication status	E-pub ahead of print - 1 Dec 2021

Keywords / Materials (for Non-textual outputs)

ammonium sulfate
chitosan
high pressure homogenisation
scale-up
Thermosynechococcus elongatus BP-1

Access to Document

10.1016/j.biortech.2021.126459Licence: Creative Commons: Attribution (CC-BY)

McCormickEtalBT2022PilotScaleProductionExtractionAndPurificationFinal published version, 3.31 MBLicence: Creative Commons: Attribution (CC-BY)

Core funding for the Wellcome Centre for Cell Biology
Marston, A.
UK-based charities
1/12/21 → 30/11/23
Project: Research
CyanoSource: A foundry generated barcoded mutant library resource for the model cyanobacterium Synechocystis sp. PCC 6803
McCormick, A.
BBSRC
28/10/19 → 28/10/22
Project: Research
Life through a Lens
Tollervey, D.
UK-based charities
1/10/11 → 31/01/13
Project: Research

Cite this

@article{19ef7b36be404ac485bb719eb916b85c,

title = "Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803",

abstract = "Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is presented. Batch cultivation without antibiotics had no impact on growth or Te-PC production and optimisation of growth conditions resulted in Te-PC contents of 75.3 ± 1.7 mg g DW-1. Wet biomass was harvested following chitosan-based flocculation with a 95 ± 1% efficiency, and Te-PC was extracted by high pressure homogenisation. Subsequent purification by heat-treatment and two-step ammonium sulfate precipitation removed chlorophyll and allophycocyanin contamination, resulting in Te-PC purities of 2.9 ± 0.7 and a mean Te-PC recovery of 84 ± 12%.",

keywords = "ammonium sulfate, chitosan, high pressure homogenisation, scale-up, Thermosynechococcus elongatus BP-1",

author = "Anton Puzorjov and Mert {\"U}nal and Wear, {Martin A} and McCormick, {Alistair J}",

year = "2021",

month = dec,

day = "1",

doi = "10.1016/j.biortech.2021.126459",

language = "English",

volume = "345",

journal = "Bioresource technology",

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TY - JOUR

T1 - Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

AU - Puzorjov, Anton

AU - Ünal, Mert

AU - Wear, Martin A

AU - McCormick, Alistair J

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is presented. Batch cultivation without antibiotics had no impact on growth or Te-PC production and optimisation of growth conditions resulted in Te-PC contents of 75.3 ± 1.7 mg g DW-1. Wet biomass was harvested following chitosan-based flocculation with a 95 ± 1% efficiency, and Te-PC was extracted by high pressure homogenisation. Subsequent purification by heat-treatment and two-step ammonium sulfate precipitation removed chlorophyll and allophycocyanin contamination, resulting in Te-PC purities of 2.9 ± 0.7 and a mean Te-PC recovery of 84 ± 12%.

AB - Phycocyanin (PC) is a soluble blue pigment-protein primarily harvested from the cyanobacterium Arthrospira platensis. PC is in high demand from several industries, but its narrow stability range limits potential applications. Here, a pilot scale (120 L total) batch production, extraction and purification process for thermostable PC (Te-PC) from a Synechocystis sp. PCC 6803 'Olive' strain expressing the PC operon cpcBACD from Thermosynechococcus elongatus BP-1 on a self-replicating vector is presented. Batch cultivation without antibiotics had no impact on growth or Te-PC production and optimisation of growth conditions resulted in Te-PC contents of 75.3 ± 1.7 mg g DW-1. Wet biomass was harvested following chitosan-based flocculation with a 95 ± 1% efficiency, and Te-PC was extracted by high pressure homogenisation. Subsequent purification by heat-treatment and two-step ammonium sulfate precipitation removed chlorophyll and allophycocyanin contamination, resulting in Te-PC purities of 2.9 ± 0.7 and a mean Te-PC recovery of 84 ± 12%.

KW - ammonium sulfate

KW - chitosan

KW - high pressure homogenisation

KW - scale-up

KW - Thermosynechococcus elongatus BP-1

U2 - 10.1016/j.biortech.2021.126459

DO - 10.1016/j.biortech.2021.126459

M3 - Article

SN - 0960-8524

VL - 345

JO - Bioresource technology

JF - Bioresource technology

M1 - 126459

ER -

Pilot scale production, extraction and purification of a thermostable phycocyanin from Synechocystis sp. PCC 6803

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

Fingerprint

Projects

Core funding for the Wellcome Centre for Cell Biology

CyanoSource: A foundry generated barcoded mutant library resource for the model cyanobacterium Synechocystis sp. PCC 6803

Life through a Lens

Cite this