TY - JOUR
T1 - Plant plasmodesmata bridges form through ER-dependent incomplete cytokinesis
AU - Li, Ziqiang P.
AU - Moreau, Hortense
AU - Petit, Jules D.
AU - Moraes, Tatiana S.
AU - Smokvarska, Marija
AU - Pérez-Sancho, Jessica
AU - Petrel, Melina
AU - Decoeur, Fanny
AU - Brocard, Lysiane
AU - Chambaud, Clément
AU - Grison, Magali S.
AU - Paterlini, Andrea
AU - Glavier, Marie
AU - Hoornaert, Lucie
AU - Joshi, Amit S.
AU - Gontier, Etienne
AU - Prinz, William A.
AU - Jaillais, Yvon
AU - Taly, Antoine
AU - Campelo, Felix
AU - Caillaud, Marie Cécile
AU - Bayer, Emmanuelle M.
N1 - We thank K. Verstaen from the Vlaams Instituut voor Biotechnologie single cell core, who provided the single-cell sequencing raw data, which we used in fig. S6. S. Otero provided the MCTP6 native reporter line. We thank S. Marais for the help with iMaris software, G. Maucort for suggestions on the SBF-SEM visualization, and F. Cordelières for live-imaging data display. We also thank M. Schuldiner, P. Bassereau, O. Hamant, A. Chaigne, T. Brunet, Y. Boutté, and S. Mongrand for reading and commenting on the article. All light and electron imaging were done at the Bordeaux Imaging Center, a member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04).
PY - 2024/11/1
Y1 - 2024/11/1
N2 - Diverging from conventional cell division models, plant cells undergo incomplete division to generate plasmodesmata communication bridges between daughter cells. Although fundamental for plant multicellularity, the molecular events leading to bridge stabilization, as opposed to severing, remain unknown. Using electron tomography, we mapped the transition from cell plate fenestrae to plasmodesmata. We show that the endoplasmic reticulum (ER) connects daughter cells across fenestrae, and as the cell plate matures, fenestrae contract, causing the plasma membrane (PM) to mold around constricted ER tubes. The ER’s presence prevents fenestrae fusion, forming plasmodesmata, whereas its absence results in closure. The ER-PM protein tethers MCTP3, MCTP4, and MCTP6 further stabilize nascent plasmodesmata during fenestrae contraction. Genetic deletion in Arabidopsis reduces plasmodesmata formation. Our findings reveal how plants undergo incomplete division to promote intercellular communication.
AB - Diverging from conventional cell division models, plant cells undergo incomplete division to generate plasmodesmata communication bridges between daughter cells. Although fundamental for plant multicellularity, the molecular events leading to bridge stabilization, as opposed to severing, remain unknown. Using electron tomography, we mapped the transition from cell plate fenestrae to plasmodesmata. We show that the endoplasmic reticulum (ER) connects daughter cells across fenestrae, and as the cell plate matures, fenestrae contract, causing the plasma membrane (PM) to mold around constricted ER tubes. The ER’s presence prevents fenestrae fusion, forming plasmodesmata, whereas its absence results in closure. The ER-PM protein tethers MCTP3, MCTP4, and MCTP6 further stabilize nascent plasmodesmata during fenestrae contraction. Genetic deletion in Arabidopsis reduces plasmodesmata formation. Our findings reveal how plants undergo incomplete division to promote intercellular communication.
UR - http://www.scopus.com/inward/record.url?scp=85208291841&partnerID=8YFLogxK
U2 - 10.1126/science.adn4630
DO - 10.1126/science.adn4630
M3 - Article
C2 - 39480927
AN - SCOPUS:85208291841
SN - 2096-5672
VL - 386
SP - 538
EP - 545
JO - Journal of Bio-X Research
JF - Journal of Bio-X Research
IS - 6721
ER -