Plasma Autoantibodies Associated with Basal-like Breast Cancers

Jie Wang, Jonine D Figueroa, Garrick Wallstrom, Kristi Barker, Jin G Park, Gokhan Demirkan, Jolanta Lissowska, Karen S Anderson, Ji Qiu, Joshua LaBaer

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Basal-like breast cancer (BLBC) is a rare aggressive subtype that is less likely to be detected through mammographic screening. Identification of circulating markers associated with BLBC could have promise in detecting and managing this deadly disease.

METHODS: Using samples from the Polish Breast Cancer study, a high-quality population-based case-control study of breast cancer, we screened 10,000 antigens on protein arrays using 45 BLBC patients and 45 controls, and identified 748 promising plasma autoantibodies (AAbs) associated with BLBC. ELISA assays of promising markers were performed on a total of 145 BLBC cases and 145 age-matched controls. Sensitivities at 98% specificity were calculated and a BLBC classifier was constructed.

RESULTS: We identified 13 AAbs (CTAG1B, CTAG2, TP53, RNF216, PPHLN1, PIP4K2C, ZBTB16, TAS2R8, WBP2NL, DOK2, PSRC1, MN1, TRIM21) that distinguished BLBC from controls with 33% sensitivity and 98% specificity. We also discovered a strong association of TP53 AAb with its protein expression (P = 0.009) in BLBC patients. In addition, MN1 and TP53 AAbs were associated with worse survival [MN1 AAb marker HR = 2.25, 95% confidence interval (CI), 1.03-4.91; P = 0.04; TP53, HR = 2.02, 95% CI, 1.06-3.85; P = 0.03]. We found limited evidence that AAb levels differed by demographic characteristics.

CONCLUSIONS: These AAbs warrant further investigation in clinical studies to determine their value for further understanding the biology of BLBC and possible detection.

IMPACT: Our study identifies 13 AAb markers associated specifically with BLBC and may improve detection or management of this deadly disease.

Original languageEnglish
Pages (from-to)1332-40
Number of pages9
JournalCancer Epidemiology, Biomarkers and Prevention
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 2015

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