Plasmacytoid dendritic cells migrate in afferent skin lymph

F. Pascale, V. Contreras, M. Bonneau, A. Courbet, S. Chilmonczyk, C. Bevilacqua, M. Eparaud, V. Niborski, S. Riffault, A.-M. Balazuc, E. Foulon, L. Guzylack-Piriou, B. Riteau, J. Hope, N. Bertho, B. Charley, I. Schwartz-Cornil

Research output: Contribution to journalArticlepeer-review

Abstract

Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid dendritic cells (pDC), also called IFN-producing cells (IPC), are described to gain nodes from blood via the high endothelial venules. We demonstrate here that IPC/pDC migrate in the afferent lymph of two large mammals. In sheep, injection of type A CpG oligodinucleotide (ODN) induced lymph cells to produce type I IFN. Furthermore, low-density lymph cells collected at steady state produced type I IFN after stimulation with type A CpG ODN and enveloped viruses. Sheep lymph IPC were found within a minor B-neg CD11c(neg) subset expressing CD45RB. They presented a plasmacytoid morphology, expressed high levels of TLR-7, TLR-9, and IFN regulatory factor 7 mRNA, induced IFN-gamma production in allogeneic CD4(pos) T cells, and differentiated into dendritic cell-like cells under viral stimulation, thus fulfilling criteria of bona fide pDC. In mini-pig, a CD4(pos)SIRP(pos) subset in afferent lymph cells, corresponding to pDC homologs, produced type I IFN after type A CpG-ODN triggering. Thus, pDC can link innate and acquired immunity by migrating from tissue to draining node via lymph, similarly to conventional dendritic cells.
Original languageUndefined/Unknown
Pages (from-to)5963-5972
Number of pages10
JournalJournal of Immunology
Volume180
Issue number9
Publication statusPublished - 2008

Keywords

  • Animals
  • Antigens, CD11c/immunology
  • Antigens, CD45/immunology
  • CD4-Positive T-Lymphocytes/cytology
  • CD4-Positive T-Lymphocytes/immunology
  • Cell Movement/drug effects
  • Cell Movement/physiology
  • Dendritic Cells
  • Female
  • Immunity, Innate
  • Interferon Regulatory Factor-7/immunology
  • Interferon Type I/immunology
  • Lymph/cytology
  • Lymph/immunology
  • Lymph Nodes
  • Oligodeoxyribonucleotides
  • Plasma Cells
  • Sheep
  • Skin/cytology
  • Skin/immunology
  • Swine
  • Swine, Miniature
  • Toll-Like Receptor 7/immunology
  • Toll-Like Receptor 9/immunology
  • Viruses/immunology

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