Abstract / Description of output
Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid dendritic cells (pDC), also called IFN-producing cells (IPC), are described to gain nodes from blood via the high endothelial venules. We demonstrate here that IPC/pDC migrate in the afferent lymph of two large mammals. In sheep, injection of type A CpG oligodinucleotide (ODN) induced lymph cells to produce type I IFN. Furthermore, low-density lymph cells collected at steady state produced type I IFN after stimulation with type A CpG ODN and enveloped viruses. Sheep lymph IPC were found within a minor B-neg CD11c(neg) subset expressing CD45RB. They presented a plasmacytoid morphology, expressed high levels of TLR-7, TLR-9, and IFN regulatory factor 7 mRNA, induced IFN-gamma production in allogeneic CD4(pos) T cells, and differentiated into dendritic cell-like cells under viral stimulation, thus fulfilling criteria of bona fide pDC. In mini-pig, a CD4(pos)SIRP(pos) subset in afferent lymph cells, corresponding to pDC homologs, produced type I IFN after type A CpG-ODN triggering. Thus, pDC can link innate and acquired immunity by migrating from tissue to draining node via lymph, similarly to conventional dendritic cells.
Original language | Undefined/Unknown |
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Pages (from-to) | 5963-5972 |
Number of pages | 10 |
Journal | The Journal of Immunology |
Volume | 180 |
Issue number | 9 |
Publication status | Published - 2008 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Antigens, CD11c/immunology
- Antigens, CD45/immunology
- CD4-Positive T-Lymphocytes/cytology
- CD4-Positive T-Lymphocytes/immunology
- Cell Movement/drug effects
- Cell Movement/physiology
- Dendritic Cells
- Female
- Immunity, Innate
- Interferon Regulatory Factor-7/immunology
- Interferon Type I/immunology
- Lymph/cytology
- Lymph/immunology
- Lymph Nodes
- Oligodeoxyribonucleotides
- Plasma Cells
- Sheep
- Skin/cytology
- Skin/immunology
- Swine
- Swine, Miniature
- Toll-Like Receptor 7/immunology
- Toll-Like Receptor 9/immunology
- Viruses/immunology