Plasmid and bacteriophage vectors for excision of intact inserts

R. Lathe*, J. L. Vilotte, A. J. Clark

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalGene
Volume57
Issue number2-3
DOIs
Publication statusPublished - 1987

Keywords

  • (Recombinant DNA
  • bacteriophage λ
  • bovine genomic library
  • plasmid
  • restriction enzymes SfiI, NotI
  • transgenic)
  • vector

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