Plasmid and bacteriophage vectors for excision of intact inserts

R. Lathe; J. L. Vilotte; A. J. Clark

doi:10.1016/0378-1119(87)90122-3

Plasmid and bacteriophage vectors for excision of intact inserts

R. Lathe^*, J. L. Vilotte, A. J. Clark

^*Corresponding author for this work

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.

Original language	English
Pages (from-to)	193-201
Number of pages	9
Journal	Gene
Volume	57
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(87)90122-3
Publication status	Published - 1987

Keywords / Materials (for Non-textual outputs)

(Recombinant DNA
bacteriophage λ
bovine genomic library
plasmid
restriction enzymes SfiI, NotI
transgenic)
vector

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10.1016/0378-1119(87)90122-3

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title = "Plasmid and bacteriophage vectors for excision of intact inserts",

abstract = "Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.",

keywords = "(Recombinant DNA, bacteriophage λ, bovine genomic library, plasmid, restriction enzymes SfiI, NotI, transgenic), vector",

author = "R. Lathe and Vilotte, {J. L.} and Clark, {A. J.}",

year = "1987",

doi = "10.1016/0378-1119(87)90122-3",

language = "English",

volume = "57",

pages = "193--201",

journal = "Gene",

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TY - JOUR

T1 - Plasmid and bacteriophage vectors for excision of intact inserts

AU - Lathe, R.

AU - Vilotte, J. L.

AU - Clark, A. J.

PY - 1987

Y1 - 1987

N2 - Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.

AB - Plasmid (pPolyIII) and bacteriophage λ (EMBL301) vectors are described in which sites for the rare-cutting enzymes SfiI and NotI (8-bp, recognition sequences) flank the Polylinker cloning region. Intact DNA inserts for introduction into cultured cells or into the early embryo are readily excised from the vectors. General-purpose miniplasmid cloning vectors pPolyI and pPolyII are also described, and the utility of the bacteriophage λ vector is demonstrated in the construction of a bovine genomic library.

KW - (Recombinant DNA

KW - bacteriophage λ

KW - bovine genomic library

KW - plasmid

KW - restriction enzymes SfiI, NotI

KW - transgenic)

KW - vector

UR - http://www.scopus.com/inward/record.url?scp=0023490560&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(87)90122-3

DO - 10.1016/0378-1119(87)90122-3

M3 - Article

C2 - 2826295

AN - SCOPUS:0023490560

SN - 0378-1119

VL - 57

SP - 193

EP - 201

JO - Gene

JF - Gene

IS - 2-3

ER -

Plasmid and bacteriophage vectors for excision of intact inserts

Abstract

Keywords / Materials (for Non-textual outputs)

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