Platelet receptor polymorphisms do not influence Staphylococcus aureus-platelet interactions or infective endocarditis

Shruti Daga, J. G. Shepherd, J. G. Callaghan, R. K. Hung, D. K. Dawson, Gareth Padfield, S. Y. Hey, R. A. Cartwright, D. E. Newby, J. R. Fitzgerald

Research output: Contribution to journalArticlepeer-review

Abstract

The germ cell lineage is a specified cell population that passes through a series of differentiation steps before giving rise, eventually, to either eggs or sperm. We have investigated the manner in which primordial germ cells (PGCs) are reprogrammed in vitro to form pluripotent stem cells in response to exogenous fibroblast growth factor-2 (FGF-2). The response is dependent on time of exposure and concentration of FGF-2. PGCs isolated in culture show a motile phenotype and lose any expression of a characteristic germ cell marker, mouse vasa homolog. Subsequently, some but not all of the cells show further changes of phenotype, accompanied by changes in expression of endogenous FGF-2 and up-regulation of its receptor, fibroblast growth factor receptor-3, in the nucleus. We propose that it is from this reprogrammed component of the now heterogeneous PGC population that pluripotent stem cells arise
Original languageEnglish
Pages (from-to)216-225
JournalMicrobes and Infection
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 2011

Keywords

  • Animals
  • cancer
  • Cell Differentiation
  • Cell Lineage
  • CELLS
  • Cells,Cultured
  • Colony-Forming Units Assay
  • cytology
  • developmental biology
  • drug effects
  • EXPRESSION
  • Female
  • Fibroblast Growth Factor 2
  • genetics
  • germ cell
  • Germ Cells
  • Growth
  • In Vitro
  • Male
  • metabolism
  • Mice
  • Mice,Inbred C57BL
  • Mice,Inbred CBA
  • MOUSE
  • nucleus
  • pharmacology
  • Phenotype
  • Pluripotent Stem Cells
  • Pregnancy
  • receptor
  • Receptor,Fibroblast Growth Factor,Type 1
  • Receptor,Fibroblast Growth Factor,Type 3
  • Research
  • Research Support
  • RNA
  • RNA,Messenger
  • Signal Transduction
  • Stem Cells
  • Up-Regulation

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