Polycomb eviction as a new distant enhancer function

Douglas Vernimmen; Magnus D. Lynch; Marco De Gobbi; David Garrick; Jacqueline A. Sharpe; Jacqueline A. Sloane-Stanley; Andrew J. H. Smith; Douglas R. Higgs

doi:10.1101/gad.16985411

Polycomb eviction as a new distant enhancer function

Douglas Vernimmen, Magnus D. Lynch, Marco De Gobbi, David Garrick, Jacqueline A. Sharpe, Jacqueline A. Sloane-Stanley, Andrew J. H. Smith, Douglas R. Higgs

Research output: Contribution to journal › Article › peer-review

Abstract

Remote distal enhancers may be located tens or thousands of kilobases away from their promoters. How they control gene expression is still poorly understood. Here, we analyze the influence of a remote enhancer on the balance between repression (Polycomb-PcG) and activation (Trithorax-TrxG) of a developmentally regulated gene associated with a CpG island. We reveal its essential, nonredundant role in clearing the PcG complex and H3K27me3 from the CpG island. In the absence of the enhancer, the H3K27me3 demethylase (JMJD3) is not recruited to the CpG island. We propose a new role of long-range regulatory elements in removing repressive PcG complexes.

Original language	English
Pages (from-to)	1583-1588
Number of pages	6
Journal	Genes & Development
Volume	25
Issue number	15
DOIs	https://doi.org/10.1101/gad.16985411
Publication status	Published - Aug 2011

Keywords / Materials (for Non-textual outputs)

polycomb
enhancer
chromatin
CpG islands

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http://genesdev.cshlp.org/content/25/15/1583

Chromatin: From Structure to Epigenetics Conference 2012, Strasbourg, 26-27th June 2012
Douglas Vernimmen (Speaker)
26 Jun 2012 → 27 Jun 2012
Activity: Participating in or organising an event types › Participation in conference
Hemoglobin Switching
Douglas Vernimmen (Participant)
7 Jun 2012 → 11 Jun 2012
Activity: Participating in or organising an event types › Participation in conference
London Chromatin Meeting
Douglas Vernimmen (Speaker)
24 Apr 2012
Activity: Participating in or organising an event types › Participation in conference

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title = "Polycomb eviction as a new distant enhancer function",

abstract = "Remote distal enhancers may be located tens or thousands of kilobases away from their promoters. How they control gene expression is still poorly understood. Here, we analyze the influence of a remote enhancer on the balance between repression (Polycomb-PcG) and activation (Trithorax-TrxG) of a developmentally regulated gene associated with a CpG island. We reveal its essential, nonredundant role in clearing the PcG complex and H3K27me3 from the CpG island. In the absence of the enhancer, the H3K27me3 demethylase (JMJD3) is not recruited to the CpG island. We propose a new role of long-range regulatory elements in removing repressive PcG complexes.",

keywords = "polycomb , enhancer, chromatin , CpG islands",

author = "Douglas Vernimmen and Lynch, {Magnus D.} and {De Gobbi}, Marco and David Garrick and Sharpe, {Jacqueline A.} and Sloane-Stanley, {Jacqueline A.} and Smith, {Andrew J. H.} and Higgs, {Douglas R.}",

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month = aug,

doi = "10.1101/gad.16985411",

language = "English",

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TY - JOUR

T1 - Polycomb eviction as a new distant enhancer function

AU - Vernimmen, Douglas

AU - Lynch, Magnus D.

AU - De Gobbi, Marco

AU - Garrick, David

AU - Sharpe, Jacqueline A.

AU - Sloane-Stanley, Jacqueline A.

AU - Smith, Andrew J. H.

AU - Higgs, Douglas R.

PY - 2011/8

Y1 - 2011/8

N2 - Remote distal enhancers may be located tens or thousands of kilobases away from their promoters. How they control gene expression is still poorly understood. Here, we analyze the influence of a remote enhancer on the balance between repression (Polycomb-PcG) and activation (Trithorax-TrxG) of a developmentally regulated gene associated with a CpG island. We reveal its essential, nonredundant role in clearing the PcG complex and H3K27me3 from the CpG island. In the absence of the enhancer, the H3K27me3 demethylase (JMJD3) is not recruited to the CpG island. We propose a new role of long-range regulatory elements in removing repressive PcG complexes.

AB - Remote distal enhancers may be located tens or thousands of kilobases away from their promoters. How they control gene expression is still poorly understood. Here, we analyze the influence of a remote enhancer on the balance between repression (Polycomb-PcG) and activation (Trithorax-TrxG) of a developmentally regulated gene associated with a CpG island. We reveal its essential, nonredundant role in clearing the PcG complex and H3K27me3 from the CpG island. In the absence of the enhancer, the H3K27me3 demethylase (JMJD3) is not recruited to the CpG island. We propose a new role of long-range regulatory elements in removing repressive PcG complexes.

KW - polycomb

KW - enhancer

KW - chromatin

KW - CpG islands

U2 - 10.1101/gad.16985411

DO - 10.1101/gad.16985411

M3 - Article

C2 - 21828268

SN - 0890-9369

VL - 25

SP - 1583

EP - 1588

JO - Genes & Development

JF - Genes & Development

IS - 15

ER -

Polycomb eviction as a new distant enhancer function

Abstract

Keywords / Materials (for Non-textual outputs)

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Activities

Chromatin: From Structure to Epigenetics Conference 2012, Strasbourg, 26-27th June 2012

Hemoglobin Switching

London Chromatin Meeting

Cite this