Polyelectrolyte Multilayer Coating of 3D Scaffolds Enhances Tissue Growth and Gene Delivery: Non-Invasive and Label-Free Assessment

Christina Holmes, Jamal Daoud, Pierre O. Bagnaninchi, Maryam Tabrizian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Layer-by-layer (LbL) deposition is a versatile technique which is beginning to be be explored for inductive tissue engineering applications. Here, it is demonstrated that a polyelectrolyte multilayer film system composed of glycol-chitosan (Glyc-CHI) and hyaluronic acid (HA) can be used to coat 3D micro-fabricated polymeric tissue engineering scaffolds. In order to overcome many of the limitations associated with conventional techniques for assessing cell growth and viability within 3D scaffolds, two novel, real-time, label-free techniques are introduced: impedance monitoring and optical coherence phase microscopy. Using these methods, it is shown that LbL-coated scaffolds support in vitro cell growth and viability for a period of at least two weeks at levels higher than uncoated controls. These polyelectrolyte multilayer coatings are then further adapted for non-viral gene delivery applications via incorporation of DNA carrier lipoplexes. Scaffold-based delivery of the enhanced green fluorescent protein (EGFP) marker gene from these coatings is successfully demonstrated in vitro, achieving a two-fold increase in transfection efficiency compared with control scaffolds. These results show the great potential of Glyc-CHI/HA polyelectrolyte multilayer films for a variety of gene delivery and inductive tissue engineering applications.

Original languageEnglish
Pages (from-to)572-580
Number of pages9
JournalAdvanced Healthcare Materials
Volume3
Issue number4
DOIs
Publication statusPublished - Apr 2014

Keywords

  • tissue engineering
  • gene delivery
  • layer-by-layer deposition
  • dielectric impedance spectroscopy
  • phase microscopy
  • HYALURONIC-ACID HYDROGELS
  • DNA DELIVERY
  • PLASMID DNA
  • CELL DIFFERENTIATION
  • POROUS SCAFFOLDS
  • FILMS
  • SURFACE
  • RELEASE
  • TRANSFECTION
  • IMPLANTS

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