Projects per year
Fluorescent particles are used for a diverse number of biochemical assays including intracellular imaging, cellular tracking, as well as detection of a variety of biomolecules. They are typically prepared by postpolymerization conjugations of dyes onto preformed particles; Herein we report the synthesis of aminomethyl-functionalized fluorescent particles via the synthesis and application of polymerizable fluorescein monomers. These monomers allowed high and controllable fluorophore loading, into the particles, resulting in enhanced fluorescence properties in comparison with, more commonly. used carboxyfluorescein conjugated particles. Furthermore, the particles were rapidly taken up by cells with enhanced fluorescence. The herein presented results demonstrate the advantages Of dye polymerization in contrast with more conventional conjugation strategies for fluorescent particle generation with applications in the life sciences.
|Number of pages||6|
|Publication status||Published - Dec 2011|
- PHOTOINDUCED ELECTRON-TRANSFER
- MINIEMULSION POLYMERIZATION
- LIVING CELLS
FingerprintDive into the research topics of 'Polymerizable Fluorescein Derivatives: Synthesis of Fluorescent Particles and Their Cellular Uptake'. Together they form a unique fingerprint.
- 2 Finished
Multimodal smart imaging of critical inflammatory processes in murine models of pulmonary fibrosis
Haslett, C., Bradley, M. & Simpson, J.
2/02/09 → 1/02/12
DEFINING THE BIOLOGICALLY EFFECTIVE DOSE
Bradley, M., Donaldson, K. & Macnee, B.
24/11/08 → 30/06/13