Population, genetic, and antigenic diversity of the apicomplexan Eimeria tenella and their relevance to vaccine development

Damer P. Blake*, Emily L. Clark, Sarah E. Macdonald, Venkatachalam Thenmozhi, Krishnendu Kundu, Rajat Garg, Isa D. Jatau, Simeon Ayoade, Fumiya Kawahara, Abdalgader Moftah, Adam James Reid, Ayotunde O. Adebambo, Ramón Álvarez Zapata, Arni S R Srinivasa Rao, Kumarasamy Thangaraj, Partha S. Banerjee, G. Dhinakar-Raj, M. Raman, Fiona M. Tomley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The phylum Apicomplexa includes serious pathogens of humans and animals. Understanding the distribution and population structure of these protozoan parasites is of fundamental importance to explain disease epidemiology and develop sustainable controls. Predicting the likely efficacy and longevity of subunit vaccines in field populations relies on knowledge of relevant preexisting antigenic diversity, population structure, the likelihood of coinfection by genetically distinct strains, and the efficiency of cross-fertilization. All four of these factors have been investigated for Plasmodium species parasites, revealing both clonal and panmictic population structures with exceptional polymorphism associated with immunoprotective antigens such as apical membrane antigen 1 (AMA1). For the coccidian Toxoplasma gondii only genomic diversity and population structure have been defined in depth so far; for the closely related Eimeria species, all four variables are currently unknown. Using Eimeria tenella, a major cause of the enteric disease coccidiosis, which exerts a profound effect on chicken productivity and welfare, we determined population structure, genotype distribution, and likelihood of crossfertilization during coinfection and also investigated the extent of naturally occurring antigenic diversity for the E. tenella AMA1 homolog. Using genome-wide Sequenom SNP-based haplotyping, targeted sequencing, and single-cell genotyping, we show that in this coccidian the functionality of EtAMA1 appears to outweigh immune evasion. This result is in direct contrast to the situation in Plasmodium and most likely is underpinned by the biology of the direct and acute coccidian life cycle in the definitive host.

Original languageEnglish
Pages (from-to)E5343-E5350
JournalProceedings of the National Academy of Sciences (PNAS)
Issue number38
Early online date9 Sept 2015
Publication statusPublished - 22 Sept 2015

Keywords / Materials (for Non-textual outputs)

  • Chickens
  • Coccidiosis
  • Eimeria
  • Food security
  • Population structure


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