Population genomics of Legionella longbeachae linked to growing media samples  reveals hidden complexities of source attribution

Rodrigo Bacigalupe; Diane Lindsay; Giles Edwards; Jonathan Fitzgerald

doi:10.3201/eid2305.161165

Population genomics of Legionella longbeachae linked to growing media samples reveals hidden complexities of source attribution

Rodrigo Bacigalupe, Diane Lindsay, Giles Edwards, Jonathan Fitzgerald

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Legionella longbeachae is the primary cause of legionellosis in Australasia and South East Asia, and an emerging pathogen in Europe and the USA. Our understanding of the population diversity of L. longbeachae from patient and environmental sources is very limited. In this study we analyzed the genomes of 70 L. longbeachae isolates, including 29 from a cluster of legionellosis cases linked to commercial growing media in Scotland in 2013, and 41 from non-outbreak isolates from Scotland and other countries. We identified extensive genetic diversity across the L. longbeachae species associated with intra- and interspecies gene flow, and a wide geographic distribution of closely related genotypes. Importantly, we observed a highly diverse pool of L. longbeachae genotypes within compost samples, which precluded the genetic establishment of an infection source. These data represent a view of the genomic diversity of the emerging pathogen L. longbeachae that will inform strategies for investigating future outbreaks.

Original language	English
Pages (from-to)	750-757
Number of pages	8
Journal	Emerging Infectious Diseases
Volume	23
Issue number	5
Early online date	20 May 2017
DOIs	https://doi.org/10.3201/eid2305.161165
Publication status	Published - May 2017

Keywords / Materials (for Non-textual outputs)

Australia
Cluster Analysis
Computational Biology
Gene Flow
Genetic Variation
Genome, Bacterial
Genomics
High-Throughput Nucleotide Sequencing
Humans
Legionella longbeachae
Legionellosis
New Zealand
Phylogeny
Plasmids
RNA, Bacterial
RNA, Ribosomal, 16S
Recombination, Genetic
Scotland
Serogroup
United States
Journal Article

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10.3201/eid2305.161165

Population Genomics of Legionella longbeachae and Hidden Complexities of Infection Source Attribution
Emerging Infectious Diseases is published by the Centers for Disease Control and Prevention, a U.S. Government agency. Therefore, materials published in Emerging Infectious Diseases, including text, figures, tables, and photographs are in the public domain and can be reprinted or used without permission with proper citation.
Final published version, 3.54 MB
16-1165
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Final published version, 3.54 MB

Genomic epidemiology of Legionnaires¿¿ disease in Scotland
Fitzgerald, R.
UK central government bodies/local authorities, health and hospital authorities
1/03/16 → 28/02/18
Project: Research
Innate immunity and endemic diseases in livestock species
Collie, D., Beard, P., Bishop, S., Bronsvoort, M., Burt, D., Fitzgerald, R., Freeman, T., Gally, D., Gill, A., Glass, E., Hocking, P., Hope, J., Hume, D., Kaiser, P., Mabbott, N., McLachlan, G., Morrison, L., Stevens, J., Stevens, M. & Watson, M.
BBSRC
1/04/12 → 31/03/17
Project: Research

Ross Fitzgerald
- Ross.Fitzgeralded.acuk
- Royal (Dick) School of Veterinary Studies - Personal Chair of Molecular Bacteriology
Person: Academic: Research Active

Cite this

@article{8312957342a94ff981c9dfa7026d1de1,

title = "Population genomics of Legionella longbeachae linked to growing media samples reveals hidden complexities of source attribution",

abstract = "Legionella longbeachae is the primary cause of legionellosis in Australasia and South East Asia, and an emerging pathogen in Europe and the USA. Our understanding of the population diversity of L. longbeachae from patient and environmental sources is very limited. In this study we analyzed the genomes of 70 L. longbeachae isolates, including 29 from a cluster of legionellosis cases linked to commercial growing media in Scotland in 2013, and 41 from non-outbreak isolates from Scotland and other countries. We identified extensive genetic diversity across the L. longbeachae species associated with intra- and interspecies gene flow, and a wide geographic distribution of closely related genotypes. Importantly, we observed a highly diverse pool of L. longbeachae genotypes within compost samples, which precluded the genetic establishment of an infection source. These data represent a view of the genomic diversity of the emerging pathogen L. longbeachae that will inform strategies for investigating future outbreaks.",

keywords = "Australia, Cluster Analysis, Computational Biology, Gene Flow, Genetic Variation, Genome, Bacterial, Genomics, High-Throughput Nucleotide Sequencing, Humans, Legionella longbeachae, Legionellosis, New Zealand, Phylogeny, Plasmids, RNA, Bacterial, RNA, Ribosomal, 16S, Recombination, Genetic, Scotland, Serogroup, United States, Journal Article",

author = "Rodrigo Bacigalupe and Diane Lindsay and Giles Edwards and Jonathan Fitzgerald",

year = "2017",

month = may,

doi = "10.3201/eid2305.161165",

language = "English",

volume = "23",

pages = "750--757",

journal = "Emerging Infectious Diseases",

issn = "1080-6040",

publisher = "Centers for Disease Control and Prevention",

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T1 - Population genomics of Legionella longbeachae linked to growing media samples reveals hidden complexities of source attribution

AU - Bacigalupe, Rodrigo

AU - Lindsay, Diane

AU - Edwards, Giles

AU - Fitzgerald, Jonathan

PY - 2017/5

Y1 - 2017/5

N2 - Legionella longbeachae is the primary cause of legionellosis in Australasia and South East Asia, and an emerging pathogen in Europe and the USA. Our understanding of the population diversity of L. longbeachae from patient and environmental sources is very limited. In this study we analyzed the genomes of 70 L. longbeachae isolates, including 29 from a cluster of legionellosis cases linked to commercial growing media in Scotland in 2013, and 41 from non-outbreak isolates from Scotland and other countries. We identified extensive genetic diversity across the L. longbeachae species associated with intra- and interspecies gene flow, and a wide geographic distribution of closely related genotypes. Importantly, we observed a highly diverse pool of L. longbeachae genotypes within compost samples, which precluded the genetic establishment of an infection source. These data represent a view of the genomic diversity of the emerging pathogen L. longbeachae that will inform strategies for investigating future outbreaks.

AB - Legionella longbeachae is the primary cause of legionellosis in Australasia and South East Asia, and an emerging pathogen in Europe and the USA. Our understanding of the population diversity of L. longbeachae from patient and environmental sources is very limited. In this study we analyzed the genomes of 70 L. longbeachae isolates, including 29 from a cluster of legionellosis cases linked to commercial growing media in Scotland in 2013, and 41 from non-outbreak isolates from Scotland and other countries. We identified extensive genetic diversity across the L. longbeachae species associated with intra- and interspecies gene flow, and a wide geographic distribution of closely related genotypes. Importantly, we observed a highly diverse pool of L. longbeachae genotypes within compost samples, which precluded the genetic establishment of an infection source. These data represent a view of the genomic diversity of the emerging pathogen L. longbeachae that will inform strategies for investigating future outbreaks.

KW - Australia

KW - Cluster Analysis

KW - Computational Biology

KW - Gene Flow

KW - Genetic Variation

KW - Genome, Bacterial

KW - Genomics

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Legionella longbeachae

KW - Legionellosis

KW - New Zealand

KW - Phylogeny

KW - Plasmids

KW - RNA, Bacterial

KW - RNA, Ribosomal, 16S

KW - Recombination, Genetic

KW - Scotland

KW - Serogroup

KW - United States

KW - Journal Article

U2 - 10.3201/eid2305.161165

DO - 10.3201/eid2305.161165

M3 - Article

C2 - 28418314

SN - 1080-6040

VL - 23

SP - 750

EP - 757

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 5

ER -

Population genomics of Legionella longbeachae linked to growing media samples reveals hidden complexities of source attribution

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

Access to Document

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Projects

Genomic epidemiology of Legionnaires¿¿ disease in Scotland

Innate immunity and endemic diseases in livestock species

Profiles

Ross Fitzgerald

Cite this