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Abstract
The Holliday junction (HJ) structure, consisting of four DNA double helices with a central branch point, is capable of switching between conformational states upon ion binding. The HJ nanoswitch described here comprises a long, dual labeled cloverleaf oligonucleotide and a short, unlabeled oligonucleotide. Fluorescent labeling with donor and acceptor dyes placed on the HJ arms of the cloverleaf strand allows the ion induced conformational switch to be detected optically using fluorescence resonance energy transfer (FRET). The influence of donor and acceptor dye location on the detection of conformational switching has been investigated using two distinct HJ structures. In addition, the effect of increasing HJ arm length in order to increase donor and acceptor dye separation has been evaluated. We report that a preferential HJ nanoswitch structure can be determined, capable of efficient detection of ion induced conformational switching.
Original language | English |
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Title of host publication | 2006 Bio- Micro- and Nanosystems Conference |
Place of Publication | NEW YORK |
Publisher | Institute of Electrical and Electronics Engineers (IEEE) |
Pages | 36 |
Number of pages | 4 |
ISBN (Print) | 978-1-4244-0056-0 |
Publication status | Published - 2006 |
Keywords
- HOLLIDAY JUNCTION
- CRYSTAL-STRUCTURE
- ENERGY-TRANSFER
- 4-WAY JUNCTION
- NANOTECHNOLOGY
- MOLECULES
- GEOMETRY
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Dive into the research topics of 'Positional characteristics of fluorophores influencing signal output of a DNA nanoswitch'. Together they form a unique fingerprint.Projects
- 1 Finished
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Beacon Project
Ghazal, P., Crain, J., Mount, A., Walton, A. & Terry, J.
UK central government bodies/local authorities, health and hospital authorities
1/04/03 → 31/03/07
Project: Research