Unbonded post-tensioned (PT) concrete slabs have been widely used in Canada and the United States since the 1960s, as they allow increased span-to-depth ratios and excellent control of deflections compared to non-prestressed reinforced concrete flexural members. The satisfactory fire performance of unbonded, PT concrete slabs in North America was established by a series of standard fire tests performed in the United States during the 1960s. However, there is a paucity of data on the effect of elevated temperatures on cold-drawn prestressing steel, both in terms of post-fire residual mechanical properties and high-temperature stress relaxation, which can lead to significant prestress loss both during and after a fire. To aid in the post-fire evaluation of PT concrete floors, a series of high-temperature residual tension tests on prestressing steel is presented, along with a second series of tests that illustrate the irrecoverable and significant loss of prestress force that may result from steel relaxation (creep) during a fire. A preliminary model is presented that can be used to predict the change in prestress force and allow for the computation of flexural capacity of a PT slab after a fire.