Pre-implantation endometrial leukocytes in women with recurrent miscarriage

S Quenby, M Bates, T Doig, J Brewster, D I Lewis-Jones, P M Johnson, G Vince

Research output: Contribution to journalArticlepeer-review

Abstract

Immunohistochemistry was used to investigate the leukocyte populations in the endometrium of women suffering recurrent miscarriage. Mid-luteal phase endometrial biopsies were taken from 22 patients with idiopathic recurrent miscarriage and from nine women with normal obstetric histories. The samples were dated histologically and stained with a panel of monoclonal antibodies to identify leukocytes. The outcome of any pregnancy in subsequent cycles following the biopsy was determined. Similar numbers of cluster designation (CD)3(+) and CD8(+) cells were seen in both groups. However, CD4(+), CD14(+), CD16(+), CD56(+) and MHC class II(+) cells were significantly higher in the recurrent miscarriage group than in the controls. Two patients had B cells (CD22(+)) in their endometrium. No CD57(+) cells were seen in the controls; however, eight of the patients had a few CD57(+) cells present. Only two patients, both from the recurrent miscarriage group, had CD69(+) leukocytes in their endometrium. Patients who had miscarriages following endometrial biopsy had significantly more CD4(+), CD8(+), CD14(+), CD16(+), and CD56(+) leukocytes in their endometrium than either those who had live births or women with proven fertility. A different population of leukocytes was found in the pre-implantation endometrium from recurrent miscarriage patients as compared to those from fertile controls. These differences were accentuated in women who had a miscarriage subsequent to the biopsy compared with those who subsequently had a live birth.

Original languageEnglish
Pages (from-to)2386-91
Number of pages6
JournalHuman Reproduction
Volume14
Issue number9
Publication statusPublished - Sep 1999

Keywords

  • Abortion, Habitual
  • Adult
  • Antibodies, Monoclonal
  • Antigens, CD14
  • Antigens, CD4
  • Antigens, CD56
  • Antigens, CD8
  • Biopsy
  • Embryonic Development
  • Endometrium
  • Female
  • Humans
  • Immunohistochemistry
  • Immunophenotyping
  • Leukocytes
  • Luteal Phase
  • Pregnancy
  • Receptors, IgG

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