Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish

C Buckley, M T Carvalho, L K Young, S A Rider, C McFadden, C Berlage, R F Verdon, J M Taylor, J M Girkin, J J Mullins

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision. The low diffraction of the Bessel beam coupled with the ability to tightly focus it through a high NA lens allowed precise, rapid targeting of subsets of cells at anatomical depth in live, developing zebrafish kidneys. We demonstrate that these specific targeting strategies significantly increase the speed of optoablation as well as fish survival.

Original languageEnglish
Pages (from-to)5096
JournalScientific Reports
Volume7
Issue number1
Early online date11 Jul 2017
DOIs
Publication statusE-pub ahead of print - 11 Jul 2017

Keywords

  • Journal Article

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