@inbook{9a9b427629314b01b174b7ab7b8afce0,
title = "Prediction of Cellular Burden with Host--Circuit Models",
abstract = "Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host--circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.",
keywords = "Cellular burden, growth models, whole-cell modelling, ene circuit design, synthetic biology, resource allocation",
author = "Evangelos-Marios Nikolados and Wei{\ss}e, {Andrea Y.} and Oyarz{\'u}n, {Diego A.}",
year = "2021",
month = jan,
day = "7",
doi = "10.1007/978-1-0716-1032-9_13",
language = "English",
isbn = "978-1-0716-1032-9",
series = "Methods in Molecular Biology",
publisher = "Springer US",
pages = "267--291",
editor = "Filippo Menolascina",
booktitle = "Synthetic Gene Circuits : Methods and Protocols",
address = "United States",
}