PREditOR: A synthetic biology approach to removing heterochromatin from cells

Oscar Molina, Mar Carmena, Isabella E. Maudlin, William C. Earnshaw

Research output: Contribution to journalArticlepeer-review

Abstract

It is widely accepted that heterochromatin is necessary to maintain genomic stability. However, direct experimental evidence supporting this is slim. Previous studies using either enzyme inhibitors, gene knockout or knockdown studies all are subject to the caveat that drugs may have off-target effects and enzymes that modify chromatin proteins to support heterochromatin formation may also have numerous other cellular targets as well. Here, we describe PREditOR (protein reading and editing of residues), a synthetic biology approach that allows us to directly remove heterochromatin from cells without either drugs or global interference with gene function. We find that removal of heterochromatin perturbs mitotic progression and causes a dramatic increase in chromosome segregation defects, possibly as a result of interfering with the normal centromeric localization of the chromosomal passenger complex.
Original languageEnglish
Pages (from-to)495-509
JournalChromosome Research
Volume24
Issue number4
DOIs
Publication statusPublished - 6 Dec 2016

Keywords

  • heterochromatin
  • , chromosome segregation
  • centromeres
  • mitosis

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