Preferential inhibition by quercetin of mitogen-stimulated thymocyte glucose transport

D A Hume, M J Weidemann, E Ferber

Research output: Contribution to journalArticlepeer-review

Abstract

The ATPase inhibitor quercetin, which inhibits tumor glycolysis, was shown to be a glucose transport inhibitor like the chemically related compound phloretin. Rat thymocyte glucose transport stimulated by the mitogens concanavalin A or ionophore A 23187 was more sensitive than unstimulated transport to quercetin inhibition. The partial inhibition of Na+-, K+- ATPase activity by quercetin observed in tumor cells was confirmed in thymocyte plasma membranes. The specific Na+-, K+- ATPase inhibitor ouabain did not mimic the effect of quercetin on mitogen-stimulated glucose transport but did reduce the effectiveness of concanavalin A as a stimulator of mitochondrial pyruvate oxidation. The results support the idea that glycolytic flux and the activity of plasma membrane ATPase are related but suggest that glucose transport, rather than the Na+-, K+-ATPase, is the rate-limiting reaction in lymphocytes.
Original languageEnglish
Pages (from-to)1243-6
Number of pages4
JournalJNCI: Journal of the National Cancer Institute
Volume62
Issue number5
Publication statusPublished - May 1979

Keywords

  • Animals
  • Biological Transport
  • Calcimycin
  • Cell Membrane
  • Concanavalin A
  • Flavonoids
  • Glucose
  • Glycolysis
  • Mitochondria
  • Mitogens
  • Ouabain
  • Pyruvates
  • Quercetin
  • Rats
  • Sodium-Potassium-Exchanging ATPase
  • T-Lymphocytes

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