Preliminary evidence that different domains are involved in cytolytic activity and receptor (cholesterol) binding in listeriolysin O, the Listeria monocytogenes thiol-activated toxin

Jose A Vazquez-Boland, L Dominguez, Elias Fernando Rodriguez-Ferri, Jose Francisco Fernández-Garayzábal, G Suarez

Research output: Contribution to journalArticlepeer-review

Abstract

The inactive truncated 52 Kilodaltons (kDa) Listeriolysin O (LLO) produced by a transposon Tn1545-induced Listeria monocytogenes non-hemolytic/avirulent mutant previously described (Gaillard et al. (1986) Infect. Immun. 52, 50-55), that lacks a 48 aminoacid fragment at the C-terminal end including the single cysteine residue essential for activity (Mengaud et al. (1988) Infect. Immun. 56, 766-772), bound to the SH-cytolysin membrane receptor cholesterol, as did the active 60 kDa toxin. These results indicate that the missing fragment is a functionally important region needed in the 60 kDa LLO to cause membrane-disruption but not to bind to cholesterol, which strongly suggests that in LLO (and presumably in the other SH-cytolysins, in accordance with their structural and functional homologies) different domains are involved in cytolytic activity and cholesterol binding. The cysteine residue contained in the missing fragment, therefore, would not be essential for cholesterol binding, as is currently thought, rather it seems to be essential specifically for cell lysis.
Original languageEnglish
Pages (from-to)95-9
Number of pages5
JournalFEMS Microbiology Letters
Volume53
Issue number1-2
DOIs
Publication statusPublished - Nov 1989

Keywords

  • Bacterial Toxins
  • Binding Sites
  • Blotting, Western
  • Cholesterol/metabolism
  • Cytotoxins/metabolism
  • Cytotoxins/pharmacology
  • Heat-Shock Proteins/metabolism
  • Heat-Shock Proteins/pharmacology
  • Hemolysin Proteins
  • Listeria monocytogenes/metabolism
  • Structure-Activity Relationship
  • Sulfhydryl Compounds/pharmacology

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