Preparation and Imaging of Specialized ER Using Super-resolution and TEM Techniques

Karen Bell, Karl Oparka, Kirsten Knox

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM) which preserve the fragile structure and allow the detailed imaging of the SER.
Original languageEnglish
Title of host publicationThe Plant Endoplasmic Reticulum
Subtitle of host publicationMethods and Protocols
EditorsChris Hawes, Verena Kriechbaumer
PublisherHumana Press
Chapter4
Pages33-42
ISBN (Electronic)978-1-4939-7389-7
ISBN (Print)978-1-4939-7388-0
DOIs
Publication statusE-pub ahead of print - 17 Oct 2017

Publication series

NameMethods in Molecular Biology

Keywords

  • 3D-SIM
  • BY2
  • Endoplasmic reticulum
  • Desmotubule
  • Sieve element reticulum
  • Imaging
  • OMX
  • ZIO

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