Preparation of organotypic hippocampal slice cultures for the study of CNS disease and damage

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Organotypic hippocampal slice cultures (OHSCs) retain in vivo-like neuronal architecture, synaptic connections, and resident cell populations but gain in vitro advantages of accessibility to experimental manipulation and observation. This chapter describes how to prepare OHSCs from neonatal mice to study mechanisms of neuronal damage, including synapse loss and quantifying Aβ-containing axonal swellings from Alzheimer’s disease transgenic mice.
Original languageEnglish
Title of host publicationAxon Degeneration
EditorsElisabetta Babetto
PublisherSpringer
Pages133-144
Number of pages11
ISBN (Electronic)978-1-0716-0584-4
ISBN (Print)978-1-0716-0584-4
Publication statusE-pub ahead of print - 11 Jun 2020

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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