Preparation of radioactively labelled isodityrosine

Janice G. Miller; Stephen C. Fry

doi:10.1002/pca.2800030204

Preparation of radioactively labelled isodityrosine

Janice G. Miller, Stephen C. Fry^*

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Oxidation of L‐[U‐¹⁴C]tyrosine with 2.7 molar equivalents of alkaline hexacyanoferrate(III) yielded at least 12 chromatographically mobile oxidation products and a large amount of immobile material. Use of 0.23 molar equivalents of hexacyanoferrate(III) yielded [¹⁴C]dityrosine (∼1.3% of added [¹⁴C]tyrosine) and [¹⁴C]isodityrosine (∼0.6%). A chromatographic method is described for the isolation of these two products from the mixture. A method is also described for the prepration of [³H]isodityrosine from non‐radioactive isodityrosine by catalytic exchange with ³H₂. The [³H]isodityrosine formed was essentially stable in 6M HCI at 110°C, indicating that the tritiation occurred at the benzylic groups.

Original language	English
Pages (from-to)	61-64
Number of pages	4
Journal	Phytochemical analysis
Volume	3
Issue number	2
DOIs	https://doi.org/10.1002/pca.2800030204
Publication status	Published - Mar 1992

Keywords / Materials (for Non-textual outputs)

dityrosine
extensin
Isodityrosine
paper chromatography
phenolic coupling
tritiation

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10.1002/pca.2800030204

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title = "Preparation of radioactively labelled isodityrosine",

abstract = "Oxidation of L‐[U‐14C]tyrosine with 2.7 molar equivalents of alkaline hexacyanoferrate(III) yielded at least 12 chromatographically mobile oxidation products and a large amount of immobile material. Use of 0.23 molar equivalents of hexacyanoferrate(III) yielded [14C]dityrosine (∼1.3% of added [14C]tyrosine) and [14C]isodityrosine (∼0.6%). A chromatographic method is described for the isolation of these two products from the mixture. A method is also described for the prepration of [3H]isodityrosine from non‐radioactive isodityrosine by catalytic exchange with 3H2. The [3H]isodityrosine formed was essentially stable in 6M HCI at 110°C, indicating that the tritiation occurred at the benzylic groups.",

keywords = "dityrosine, extensin, Isodityrosine, paper chromatography, phenolic coupling, tritiation",

author = "Miller, {Janice G.} and Fry, {Stephen C.}",

year = "1992",

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doi = "10.1002/pca.2800030204",

language = "English",

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journal = "Phytochemical analysis",

issn = "0958-0344",

publisher = "Wiley",

number = "2",

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TY - JOUR

T1 - Preparation of radioactively labelled isodityrosine

AU - Miller, Janice G.

AU - Fry, Stephen C.

PY - 1992/3

Y1 - 1992/3

N2 - Oxidation of L‐[U‐14C]tyrosine with 2.7 molar equivalents of alkaline hexacyanoferrate(III) yielded at least 12 chromatographically mobile oxidation products and a large amount of immobile material. Use of 0.23 molar equivalents of hexacyanoferrate(III) yielded [14C]dityrosine (∼1.3% of added [14C]tyrosine) and [14C]isodityrosine (∼0.6%). A chromatographic method is described for the isolation of these two products from the mixture. A method is also described for the prepration of [3H]isodityrosine from non‐radioactive isodityrosine by catalytic exchange with 3H2. The [3H]isodityrosine formed was essentially stable in 6M HCI at 110°C, indicating that the tritiation occurred at the benzylic groups.

AB - Oxidation of L‐[U‐14C]tyrosine with 2.7 molar equivalents of alkaline hexacyanoferrate(III) yielded at least 12 chromatographically mobile oxidation products and a large amount of immobile material. Use of 0.23 molar equivalents of hexacyanoferrate(III) yielded [14C]dityrosine (∼1.3% of added [14C]tyrosine) and [14C]isodityrosine (∼0.6%). A chromatographic method is described for the isolation of these two products from the mixture. A method is also described for the prepration of [3H]isodityrosine from non‐radioactive isodityrosine by catalytic exchange with 3H2. The [3H]isodityrosine formed was essentially stable in 6M HCI at 110°C, indicating that the tritiation occurred at the benzylic groups.

KW - dityrosine

KW - extensin

KW - Isodityrosine

KW - paper chromatography

KW - phenolic coupling

KW - tritiation

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U2 - 10.1002/pca.2800030204

DO - 10.1002/pca.2800030204

M3 - Article

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SN - 0958-0344

VL - 3

SP - 61

EP - 64

JO - Phytochemical analysis

JF - Phytochemical analysis

IS - 2

ER -

Preparation of radioactively labelled isodityrosine

Abstract

Keywords / Materials (for Non-textual outputs)

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