Prevalence and epidemiology of non-O157 Escherichia coli serogroups O26, O103, O111, O145 and Shiga toxin gene carriage in Scottish cattle, 2014-2015.

Deborah Hoyle, Marianne Keith, Helen Williamson, Kareen Macleod, Heather Mathie, Ian Handel, Carol Currie, Anne Holmes, Lesley Allison, Becca McLean, Rebecca Callaby, Thibaud Porphyre, Sue C Tongue, Madeleine K. Henry, Judith Evans, George J. Gunn, David Gally, Nuno A Silva, Margo Chase-Topping

Research output: Contribution to journalArticlepeer-review

Abstract

Cattle are a reservoir for Shiga toxin-producing Escherichia coli (STEC), zoonotic
pathogens that cause serious clinical disease. Scotland has a higher incidence of
STEC infection in the human population than the European average. The aim of this study was to investigate the prevalence and epidemiology of non-O157 serogroups O26, O103, O111, O145, and Shiga toxin gene carriage, in Scottish cattle. Faecal samples (n = 2783) were collected from 110 herds between 2014-2015 and screened by real-time PCR. Herd-level prevalence (95% CI) for O103, O26 andO145 was estimated as 0.71 (0.62, 0.79), 0.43 (0.34, 0.52) and 0.23 (0.16, 0.32), respectively. Only two herds were positive for O111. Shiga toxin prevalence was high in both herds and pats, particularly for stx2 (herd-level: 0.99, 95% CI: 0.94, 1.0). O26 bacterial strains were isolated from 36 herds on culture. Fifteen herds yielded O26 stx-positive isolates that additionally harboured the intimin gene; six of these herds shed highly pathogenic stx2a-positive strains. Multiple serogroups were detected in herds and pats, with only 25 herds negative for all serogroups. Despite overlap in detection, regional and seasonal effects were observed. Higher herd prevalence for O26, O103 and stx1 occurred in the South West and this region was significant for stx2 at the pat-level (P = 0.015). Significant seasonal variation was observed for O145 prevalence, highest in autumn (P = 0.032). Negative herds were associated with Central Scotland and winter. Herds positive for all serogroups were associated with autumn, larger herd size and were not housed at sampling.
Original languageEnglish
JournalApplied and Environmental Microbiology
Early online date27 Apr 2021
DOIs
Publication statusE-pub ahead of print - 27 Apr 2021

Keywords

  • Shiga toxin-producing Escherichia coli,
  • non-O157
  • cattle
  • epidemiology

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