Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation

Alison M Condliffe; Phillip T Hawkins; Len R Stephens; Christopher Haslett; Edwin R Chilvers

doi:10.1016/S0014-5793(98)01358-1

Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation

Alison M Condliffe
, Phillip T Hawkins
, Len R Stephens
, Christopher Haslett
, Edwin R Chilvers

School of Regeneration and Repair

Research output: Contribution to journal › Article › peer-review

Abstract

In human neutrophils, significant agonist-stimulated superoxide anion (O2-) release is observed only after exposure to a priming agent such as TNFalpha. We have investigated the potential for TNFalpha to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P3 and PtdIns(3,4,5)P3 accumulation. TNFalpha pretreatment did not affect basal or stimulated Ins(1,4,5)P3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O2- generation implying a possible role for PtdIns(3,4,5)P3 in signalling primed O2- release.

Original language	English
Pages (from-to)	147-51
Number of pages	5
Journal	FEBS Letters
Volume	439
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)01358-1
Publication status	Published - Nov 1998

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@article{69ac4f941eec4d6085d3c9a991a56460,

title = "Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation",

abstract = "In human neutrophils, significant agonist-stimulated superoxide anion (O2-) release is observed only after exposure to a priming agent such as TNFalpha. We have investigated the potential for TNFalpha to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P3 and PtdIns(3,4,5)P3 accumulation. TNFalpha pretreatment did not affect basal or stimulated Ins(1,4,5)P3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O2- generation implying a possible role for PtdIns(3,4,5)P3 in signalling primed O2- release.",

author = "Condliffe, \{Alison M\} and Hawkins, \{Phillip T\} and Stephens, \{Len R\} and Christopher Haslett and Chilvers, \{Edwin R\}",

year = "1998",

month = nov,

doi = "10.1016/S0014-5793(98)01358-1",

language = "English",

volume = "439",

pages = "147--51",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "1-2",

}

Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation. / Condliffe, Alison M; Hawkins, Phillip T; Stephens, Len R et al.
In: FEBS Letters, Vol. 439, No. 1-2, 11.1998, p. 147-51.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation

AU - Condliffe, Alison M

AU - Hawkins, Phillip T

AU - Stephens, Len R

AU - Haslett, Christopher

AU - Chilvers, Edwin R

PY - 1998/11

Y1 - 1998/11

N2 - In human neutrophils, significant agonist-stimulated superoxide anion (O2-) release is observed only after exposure to a priming agent such as TNFalpha. We have investigated the potential for TNFalpha to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P3 and PtdIns(3,4,5)P3 accumulation. TNFalpha pretreatment did not affect basal or stimulated Ins(1,4,5)P3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O2- generation implying a possible role for PtdIns(3,4,5)P3 in signalling primed O2- release.

AB - In human neutrophils, significant agonist-stimulated superoxide anion (O2-) release is observed only after exposure to a priming agent such as TNFalpha. We have investigated the potential for TNFalpha to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P3 and PtdIns(3,4,5)P3 accumulation. TNFalpha pretreatment did not affect basal or stimulated Ins(1,4,5)P3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O2- generation implying a possible role for PtdIns(3,4,5)P3 in signalling primed O2- release.

U2 - 10.1016/S0014-5793(98)01358-1

DO - 10.1016/S0014-5793(98)01358-1

M3 - Article

SN - 0014-5793

VL - 439

SP - 147

EP - 151

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Priming of human neutrophil superoxide generation by tumour necrosis factor-alpha is signalled by enhanced phosphatidylinositol 3,4,5-trisphosphate but not inositol 1,4,5-trisphosphate accumulation

Abstract

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