Prion protein expression in different species: analysis with a panel of new mAbs

Gianluigi Zanusso, Dacai Liu, Sergio Ferrari, Ivan Hegyi, Xinghua Yin, Adriano Aguzzi, Simone Hornemann, Susanne Liemann, Rudi Glockshuber, Jean Manson, Paul Brown, Robert B Petersen, Pierluigi Gambetti, Man-Sun Sy

Research output: Contribution to journalArticlepeer-review

Abstract

By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.
Original languageEnglish
Pages (from-to)8812-6
Number of pages5
JournalProceedings of the National Academy of Sciences
Volume95
Issue number15
Publication statusPublished - 21 Jul 1998

Keywords

  • Animals
  • Antibodies, Monoclonal/immunology
  • Epitopes/immunology
  • Humans
  • Immunohistochemistry
  • Microscopy, Electron
  • Prions/genetics
  • Prions/immunology
  • Species Specificity
  • Tumor Cells, Cultured

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