Prion protein expression in different species: analysis with a panel of new mAbs

Gianluigi Zanusso; Dacai Liu; Sergio Ferrari; Ivan Hegyi; Xinghua Yin; Adriano Aguzzi; Simone Hornemann; Susanne Liemann; Rudi Glockshuber; Jean Manson; Paul Brown; Robert B Petersen; Pierluigi Gambetti; Man-Sun Sy

Prion protein expression in different species: analysis with a panel of new mAbs

Gianluigi Zanusso, Dacai Liu, Sergio Ferrari, Ivan Hegyi, Xinghua Yin, Adriano Aguzzi, Simone Hornemann, Susanne Liemann, Rudi Glockshuber, Jean Manson, Paul Brown, Robert B Petersen, Pierluigi Gambetti, Man-Sun Sy

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

Original language	English
Pages (from-to)	8812-6
Number of pages	5
Journal	Proceedings of the National Academy of Sciences (PNAS)
Volume	95
Issue number	15
Publication status	Published - 21 Jul 1998

Keywords

Animals
Antibodies, Monoclonal/immunology
Epitopes/immunology
Humans
Immunohistochemistry
Microscopy, Electron
Prions/genetics
Prions/immunology
Species Specificity
Tumor Cells, Cultured

Access to Document

Prion protein expression in different species - analysis with a panel of new mAbs
© 1998, The National Academy of Sciences
Final published version, 295 KB

Cite this

@article{238ef45922dc476e95fd603dfbd822c9,

title = "Prion protein expression in different species: analysis with a panel of new mAbs",

abstract = "By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.",

keywords = "Animals, Antibodies, Monoclonal/immunology, Epitopes/immunology, Humans, Immunohistochemistry, Microscopy, Electron, Prions/genetics, Prions/immunology, Species Specificity, Tumor Cells, Cultured",

author = "Gianluigi Zanusso and Dacai Liu and Sergio Ferrari and Ivan Hegyi and Xinghua Yin and Adriano Aguzzi and Simone Hornemann and Susanne Liemann and Rudi Glockshuber and Jean Manson and Paul Brown and Petersen, {Robert B} and Pierluigi Gambetti and Man-Sun Sy",

year = "1998",

month = jul,

day = "21",

language = "English",

volume = "95",

pages = "8812--6",

journal = "Proceedings of the National Academy of Sciences (PNAS)",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "15",

}

TY - JOUR

T1 - Prion protein expression in different species: analysis with a panel of new mAbs

AU - Zanusso, Gianluigi

AU - Liu, Dacai

AU - Ferrari, Sergio

AU - Hegyi, Ivan

AU - Yin, Xinghua

AU - Aguzzi, Adriano

AU - Hornemann, Simone

AU - Liemann, Susanne

AU - Glockshuber, Rudi

AU - Manson, Jean

AU - Brown, Paul

AU - Petersen, Robert B

AU - Gambetti, Pierluigi

AU - Sy, Man-Sun

PY - 1998/7/21

Y1 - 1998/7/21

N2 - By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

AB - By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

KW - Animals

KW - Antibodies, Monoclonal/immunology

KW - Epitopes/immunology

KW - Humans

KW - Immunohistochemistry

KW - Microscopy, Electron

KW - Prions/genetics

KW - Prions/immunology

KW - Species Specificity

KW - Tumor Cells, Cultured

M3 - Article

VL - 95

SP - 8812

EP - 8816

JO - Proceedings of the National Academy of Sciences (PNAS)

JF - Proceedings of the National Academy of Sciences (PNAS)

SN - 0027-8424

IS - 15

ER -

Prion protein expression in different species: analysis with a panel of new mAbs

Abstract

Keywords

Access to Document

Fingerprint

Cite this