Abstract / Description of output
Recent discoveries have implicated the gut microbiome in the progression and severity of Parkinson’s disease; however, how gut bacteria affect such neurodegenerative disorders remains unclear. Here, we report that the Bacillus subtilis probiotic strain PXN21 inhibits α-synuclein aggregation and clears preformed aggregates in an established Caenorhabditis elegans model of synucleinopathy. This protection is seen in young and aging animals and is partly mediated by DAF-16. Multiple B. subtilis strains trigger the protective effect via both spores and vegetative cells, partly due to a biofilm formation in the gut of the worms and the release of bacterial metabolites. We identify several host metabolic pathways differentially regulated in response to probiotic exposure, including sphingolipid metabolism. We further demonstrate functional roles of the sphingolipid metabolism genes lagr-1, asm-3, and sptl-3 in the anti-aggregation effect. Our findings provide a basis for exploring the disease-modifying potential of B. subtilis as a dietary supplement.
Original language | English |
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Pages (from-to) | 367-380.e7 |
Number of pages | 21 |
Journal | Cell Reports |
Volume | 30 |
Issue number | 2 |
DOIs | |
Publication status | Published - 14 Jan 2020 |
Keywords / Materials (for Non-textual outputs)
- probiotics
- B. subtilis
- C. elegans
- α-synuclein
- microbiota
- Parkinson’s disease
- DAF-16/FOXO
- dietary restriction
- sphingolipid metabolism
- biofilm
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Dive into the research topics of 'Probiotic Bacillus subtilis Protects against α-Synuclein Aggregation in C. elegans'. Together they form a unique fingerprint.Datasets
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Probiotic Bacillus subtilis Protects against a-Synuclein Aggregation in C. elegans (Goya ME et al., Cell Reports, 2020)
Goya, E. (Creator) & Romanowski, A. (Depositor), The University of Edinburgh, 10 Aug 2020
https://publicomero.bio.ed.ac.uk/webclient/?show=project-58
Dataset
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E-MTAB-8164 - RNAseq of young adult C. elegans animals fed on three different bacterial diets, E. coli OP50, B. subtilis PXN21 and a mixture of both
Goya, E. (Creator), Romanowski, A. (Depositor) & Doitsidou, M. (Project Leader), ArrayExpress Archive of Functional Genomics Data, 14 Jan 2020
https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-8164
Dataset
Profiles
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Maria Doitsidou
- Deanery of Biomedical Sciences - Senior Lecturer
- Centre for Discovery Brain Sciences
- Edinburgh Neuroscience
Person: Academic: Research Active