Projects per year
Abstract
Macrophages contribute to innate and acquired immunity as well as many aspects of homeostasis and development. Studies of macrophage biology and function in birds have been hampered by a lack of definitive cell surface markers. As in mammals, avian macrophages proliferate and differentiate in response to CSF1 and IL34, acting through the shared receptor, CSF1R. CSF1R mRNA expression in the chicken is restricted to macrophages and their progenitors. To expedite studies of avian macrophage biology, we produced an avian CSF1R-Fc chimeric protein and generated a monoclonal antibody (designated ROS-AV170) against the chicken CSF1R using the chimeric protein as immunogen. Specific binding of ROS-AV170 to CSF1R was confirmed by FACS, ELISA and immunohistochemistry on tissue sections. CSF1 down-regulated cell surface expression of the CSF1R detected with ROS-AV170, but the antibody did not block CSF1 signalling. Expression of CSF1R was detected on the surface of bone marrow progenitors only after culture in the absence of CSF1, and was induced during macrophage differentiation. Constitutive surface expression of CSF1R distinguished monocytes from other myeloid cells, including heterophils and thrombocytes. This antibody will therefore be of considerable utility for the study of chicken macrophage biology. Abreviations: mAb, monoclonal antibody; BMDM, bone marrow-derived macrophages; CSF1R, colony stimulating factor 1 receptor; FACS, fluorescence-activated cell sorting; PBMC, peripheral blood monocytes; WB, western blot.
| Original language | English |
|---|---|
| Pages (from-to) | 278-285 |
| Journal | Developmental and Comparative Immunology |
| Volume | 42 |
| Issue number | 2 |
| Early online date | 29 Sept 2013 |
| DOIs | |
| Publication status | Published - Jan 2014 |
Keywords / Materials (for Non-textual outputs)
- Antibody
- bone marrow-derived macrophages
- colony stimulating factor 1 receptor
- fluorescence-activated cell sorting
- Macrophages
- peripheral blood monocytes
- Western blot
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Dive into the research topics of 'Production and characterisation of a monoclonal antibody that recognises the chicken CSF1 receptor and confirms that expression is restricted to macrophage-lineage cells'. Together they form a unique fingerprint.Projects
- 3 Finished
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Innate immunity and endemic diseases in livestock species
Collie, D. (Principal Investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Fitzgerald, R. (Co-investigator), Freeman, T. (Co-investigator), Gally, D. (Co-investigator), Gill, A. (Co-investigator), Glass, E. (Co-investigator), Hocking, P. (Co-investigator), Hope, J. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Mabbott, N. (Co-investigator), McLachlan, G. (Co-investigator), Morrison, L. (Co-investigator), Stevens, J. (Co-investigator), Stevens, M. (Co-investigator) & Watson, M. (Co-investigator)
1/04/12 → 31/03/17
Project: Research
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Development of applications of CSF-1 and IL34 in livestock
Hume, D. (Principal Investigator), Gill, A. (Co-investigator) & Sang, H. (Co-investigator)
16/01/12 → 31/03/15
Project: Research
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CSF1 IL34 and the function of macrophages in avain development
Hume, D. (Principal Investigator), Kaiser, P. (Co-investigator) & Sang, H. (Co-investigator)
1/06/10 → 11/08/13
Project: Research