Abstract
Using genetic immunisation of mice, we produced antibodies against chicken interleukin-2 (ChIL-2), the first produced against a non-mammalian interleukin. After a final injection with a recombinant ChIL-2 protein, two stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of the two MAbs to recombinant ChIL-2 produced by Escherichia coli and COS-7 cells was demonstrated in an indirect ELISA, Western blotting and dot blots. Both of them were able to neutralise the biological activity of the ChIL-2, but neither allowed the detection of ChIL-2 by flow cytometry.
| Original language | English |
|---|---|
| Pages (from-to) | 149-161 |
| Number of pages | 12 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 83 |
| Issue number | 3-4 |
| Publication status | Published - 2001 |
Keywords / Materials (for Non-textual outputs)
- interleukin-2
- Chicken
- monoclonal antibodies
- Species-specificity
- Neutralising