Production and characterisation of monoclonal antibodies specific for chicken interleukin-2

L Rothwell, A Hamblin, P Kaiser

Research output: Contribution to journalArticlepeer-review

Abstract

Using genetic immunisation of mice, we produced antibodies against chicken interleukin-2 (ChIL-2), the first produced against a non-mammalian interleukin. After a final injection with a recombinant ChIL-2 protein, two stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of the two MAbs to recombinant ChIL-2 produced by Escherichia coli and COS-7 cells was demonstrated in an indirect ELISA, Western blotting and dot blots. Both of them were able to neutralise the biological activity of the ChIL-2, but neither allowed the detection of ChIL-2 by flow cytometry.
Original languageEnglish
Pages (from-to)149-161
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume83
Issue number3-4
Publication statusPublished - 2001

Keywords

  • interleukin-2
  • Chicken
  • monoclonal antibodies
  • Species-specificity
  • Neutralising

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