Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

Janice G. Miller*, Stephen C. Fry

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Cell-suspension cultures of Spinacia and Rosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures of Lycopersicon, Capsicum, Acer and Festuca did not accumulate this class of extensin. In Spinacia and Rosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume31
Issue number1
DOIs
Publication statusPublished - 1 Oct 1992

Keywords

  • cell wall-bound protein
  • extensin
  • Rosa sp.
  • salt-leaching
  • Spinacia oleracea
  • yield

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