Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

Janice G. Miller; Stephen C. Fry

doi:10.1007/BF00043476

Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

Janice G. Miller^*, Stephen C. Fry

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Cell-suspension cultures of Spinacia and Rosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures of Lycopersicon, Capsicum, Acer and Festuca did not accumulate this class of extensin. In Spinacia and Rosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl₂. Older cultures, pH values >6.5, and LaCl₃ or higher concentrations of CaCl₂ were less effective.

Original language	English
Pages (from-to)	61-66
Number of pages	6
Journal	Plant Cell, Tissue and Organ Culture
Volume	31
Issue number	1
DOIs	https://doi.org/10.1007/BF00043476
Publication status	Published - 1 Oct 1992

Keywords / Materials (for Non-textual outputs)

cell wall-bound protein
extensin
Rosa sp.
salt-leaching
Spinacia oleracea
yield

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10.1007/BF00043476

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title = "Production and harvesting of ionically wall-bound extensin from living cell suspension cultures",

abstract = "Cell-suspension cultures of Spinacia and Rosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures of Lycopersicon, Capsicum, Acer and Festuca did not accumulate this class of extensin. In Spinacia and Rosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.",

keywords = "cell wall-bound protein, extensin, Rosa sp., salt-leaching, Spinacia oleracea, yield",

author = "Miller, {Janice G.} and Fry, {Stephen C.}",

year = "1992",

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doi = "10.1007/BF00043476",

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TY - JOUR

T1 - Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

AU - Miller, Janice G.

AU - Fry, Stephen C.

PY - 1992/10/1

Y1 - 1992/10/1

N2 - Cell-suspension cultures of Spinacia and Rosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures of Lycopersicon, Capsicum, Acer and Festuca did not accumulate this class of extensin. In Spinacia and Rosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.

AB - Cell-suspension cultures of Spinacia and Rosa accumulated a cell wall protein, extensin, in a form that was amenable to leaching from the surface of the living cells by a brief treatment with non-toxic salts. Cultures of Lycopersicon, Capsicum, Acer and Festuca did not accumulate this class of extensin. In Spinacia and Rosa, optimum yields of leachable extensin were achieved from young cultures, in media at relatively low pH, by leaching with 0.1 M CaCl2. Older cultures, pH values >6.5, and LaCl3 or higher concentrations of CaCl2 were less effective.

KW - cell wall-bound protein

KW - extensin

KW - Rosa sp.

KW - salt-leaching

KW - Spinacia oleracea

KW - yield

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U2 - 10.1007/BF00043476

DO - 10.1007/BF00043476

M3 - Article

AN - SCOPUS:0002636815

SN - 0167-6857

VL - 31

SP - 61

EP - 66

JO - Plant Cell, Tissue and Organ Culture

JF - Plant Cell, Tissue and Organ Culture

IS - 1

ER -

Production and harvesting of ionically wall-bound extensin from living cell suspension cultures

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Keywords / Materials (for Non-textual outputs)

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