Production of kidney organoids arranged around single ureteric bud trees, and containing endogenous blood vessels, solely from embryonic stem cells

Anwar A. Palakkan, Julia Tarnick, Martin Waterfall, May Sallam, Fokion Glykofrydis, Mona Elhendawi, Jamie A. Davies

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

There is intense worldwide effort in generating kidney organoids from pluripotent stem cells, for research, for disease modelling and, perhaps, for making transplantable organs. Organoids generated from pluripotent stem cells (PSC) possess accurate micro-anatomy, but they lack higher-organization. This is a problem, especially for transplantation, as such organoids will not be able to perform their physiological functions. In this study, we develop a method for generating murine kidney organoids with improved higher-order structure, through stages using chimaeras of ex-fetu and PSC-derived cells to a system that works entirely from embryonic stem cells. These organoids have nephrons organised around a single ureteric bud tree and also make vessels, with the endothelial network approaching podocytes.
Original languageEnglish
Article number12573
Number of pages16
JournalScientific Reports
Volume12
Issue number1
DOIs
Publication statusPublished - 22 Jul 2022

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