Profiling of MicroRNAs in the Biofluids of Livestock Species

Jason Ioannidis, Judith Risse, F Xavier Donadeu

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

The value of circulating microRNAs (miRNAs) as noninvasive biomarkers of human disease has been extensively demonstrated. Significant potential also exists in other species, particularly in relation to control of veterinary diseases and selection/monitoring of production traits in livestock. Although robust protocols have been developed for miRNA profiling of human biofluids, significant optimization may be required before these can be applied to other species. In this chapter, we describe protocols for small-RNA sequencing and RT-qPCR analyses of plasma samples from livestock species. In addition, we provide brief data analysis protocols for small-RNA sequencing and RT-qPCR data. Finally, we highlight important considerations for these protocols such as low RNA yield, platform-specific biases, and optimal normalization approaches.

Original languageEnglish
Title of host publicationMicroRNA Protocols
PublisherSpringer Press
Pages65-77
Number of pages13
Volume1733
DOIs
Publication statusE-pub ahead of print - 13 Feb 2018

Publication series

NameMethods in Molecular Biology

Keywords

  • Cow
  • miRNA
  • microRNA
  • Biomarker
  • Plasma
  • Follicular fluid
  • Sequencing

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