Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex

Geert Hamer, Hong Wang, Ewelina Bolcun-Filas, Howard J Cooke, Ricardo Benavente, Christer Höög

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of the synaptonemal complex in Tex12-/- meiocytes revealed a disrupted central element structure, a dense structure residing between the synapsed homologous chromosomes. Chromosome synapsis is initiated at multiple positions along the paired homologous chromosomes in Tex12-/- meiotic cells, but fails to propagate along the chromosomes. Furthermore, although meiotic recombination is initiated in Tex12-/- meiotic cells, these early recombination events do not develop into meiotic crossovers. Hence, the mere initiation of synapsis is not sufficient to support meiotic crossing-over. Our results show that TEX12 is a component of the central element structure of the synaptonemal complex required for propagation of synapsis along the paired homologous chromosomes and maturation of early recombination events into crossovers.
Original languageEnglish
Pages (from-to)2445-51
Number of pages7
JournalJournal of Cell Science
Volume121
Issue numberPt 15
DOIs
Publication statusPublished - 2008

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Chromosomal Proteins, Non-Histone
  • Crossing Over, Genetic
  • DNA Breaks, Double-Stranded
  • Immunohistochemistry
  • Meiosis
  • Mice
  • Mice, Transgenic
  • Recombination, Genetic
  • Synaptonemal Complex

Fingerprint

Dive into the research topics of 'Progression of meiotic recombination requires structural maturation of the central element of the synaptonemal complex'. Together they form a unique fingerprint.

Cite this