Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA

Cristina L Esteves; Val Kelly; Amandine Breton; Ashley I Taylor; Christopher C West; Francesc X Donadeu; Bruno Péault; Jonathan R Seckl; Karen E Chapman

doi:10.1210/jc.2013-1708

Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA

Cristina L Esteves, Val Kelly, Amandine Breton, Ashley I Taylor, Christopher C West, Francesc X Donadeu, Bruno Péault, Jonathan R Seckl, Karen E Chapman

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Context:Levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which regenerates active glucocorticoids, are selectively elevated in adipose tissue in human obesity and metabolic syndrome, both conditions associated with chronic low-grade inflammation. 11β-HSD1 expression is induced by proinflammatory cytokines in a variety of cell types, including in human adipocytes differentiated in vitro.Objective:Our objective was to determine the mechanisms by which proinflammatory cytokines induce 11β-HSD1 in human adipocytes.Results:The proinflammatory cytokines IL-1α (10 ng/mL) and TNFα (20 ng/mL) increased 11β-HSD1 mRNA levels in human primary adipocyte fractions and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes (P

Original language	English
Pages (from-to)	E160-E168
Journal	The Journal of Clinical Endocrinology & Metabolism (JCEM)
Volume	99
Issue number	1
Early online date	15 Nov 2013
DOIs	https://doi.org/10.1210/jc.2013-1708
Publication status	Published - 2014

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10.1210/jc.2013-1708

http://jcem.endojournals.org/content/early/2013/11/15/jc.2013-1708

CLINCIAL ACADEMIC TRAINING F/SHIP MR CHRISTOPHER WEST : DEVELOPMENT OF 3-D POLYMER SCAFFOLD THAT PROMOTES CHONDROGENESIS OF HUMAN PERIVASCULAR STEM CELLS
West, C., Hay, D. & Peault, B.
UK central government bodies/local authorities, health and hospital authorities
1/08/11 → 31/07/14
Project: Research

Cristina Esteves
- Cristina.Estevesroslin.ed.acuk
- Royal (Dick) School of Veterinary Studies - Core Scientist (Research Fellow)
Person: Academic: Research Active (Research Assistant)

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Esteves, C. L., Kelly, V., Breton, A., Taylor, A. I., West, C. C., Donadeu, F. X., Péault, B., Seckl, J. R., & Chapman, K. E. (2014). Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA. The Journal of Clinical Endocrinology & Metabolism (JCEM), 99(1), E160-E168. https://doi.org/10.1210/jc.2013-1708

@article{b1537bb4ad7246f3ba94e471e4e201f3,

title = "Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA",

abstract = "Context:Levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which regenerates active glucocorticoids, are selectively elevated in adipose tissue in human obesity and metabolic syndrome, both conditions associated with chronic low-grade inflammation. 11β-HSD1 expression is induced by proinflammatory cytokines in a variety of cell types, including in human adipocytes differentiated in vitro.Objective:Our objective was to determine the mechanisms by which proinflammatory cytokines induce 11β-HSD1 in human adipocytes.Results:The proinflammatory cytokines IL-1α (10 ng/mL) and TNFα (20 ng/mL) increased 11β-HSD1 mRNA levels in human primary adipocyte fractions and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes (P ",

author = "Esteves, {Cristina L} and Val Kelly and Amandine Breton and Taylor, {Ashley I} and West, {Christopher C} and Donadeu, {Francesc X} and Bruno P{\'e}ault and Seckl, {Jonathan R} and Chapman, {Karen E}",

note = "This work was supported by a Wellcome Trust Programme Grant (WT083184). C.C.W. is funded by a Chief Scientist Office Clinical Academic Training Fellowship (CAF/11/13) and a research grant from the William Rooney Plastic Surgery and Burns Research Trust.",

year = "2014",

doi = "10.1210/jc.2013-1708",

language = "English",

volume = "99",

pages = "E160--E168",

journal = "The Journal of Clinical Endocrinology & Metabolism (JCEM)",

issn = "0021-972X",

publisher = "Oxford University Press",

number = "1",

}

Esteves, CL, Kelly, V, Breton, A, Taylor, AI, West, CC, Donadeu, FX, Péault, B, Seckl, JR & Chapman, KE 2014, 'Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA', The Journal of Clinical Endocrinology & Metabolism (JCEM), vol. 99, no. 1, pp. E160-E168. https://doi.org/10.1210/jc.2013-1708

Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA. / Esteves, Cristina L; Kelly, Val; Breton, Amandine et al.
In: The Journal of Clinical Endocrinology & Metabolism (JCEM), Vol. 99, No. 1, 2014, p. E160-E168.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA

AU - Esteves, Cristina L

AU - Kelly, Val

AU - Breton, Amandine

AU - Taylor, Ashley I

AU - West, Christopher C

AU - Donadeu, Francesc X

AU - Péault, Bruno

AU - Seckl, Jonathan R

AU - Chapman, Karen E

N1 - This work was supported by a Wellcome Trust Programme Grant (WT083184). C.C.W. is funded by a Chief Scientist Office Clinical Academic Training Fellowship (CAF/11/13) and a research grant from the William Rooney Plastic Surgery and Burns Research Trust.

PY - 2014

Y1 - 2014

N2 - Context:Levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which regenerates active glucocorticoids, are selectively elevated in adipose tissue in human obesity and metabolic syndrome, both conditions associated with chronic low-grade inflammation. 11β-HSD1 expression is induced by proinflammatory cytokines in a variety of cell types, including in human adipocytes differentiated in vitro.Objective:Our objective was to determine the mechanisms by which proinflammatory cytokines induce 11β-HSD1 in human adipocytes.Results:The proinflammatory cytokines IL-1α (10 ng/mL) and TNFα (20 ng/mL) increased 11β-HSD1 mRNA levels in human primary adipocyte fractions and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes (P

AB - Context:Levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which regenerates active glucocorticoids, are selectively elevated in adipose tissue in human obesity and metabolic syndrome, both conditions associated with chronic low-grade inflammation. 11β-HSD1 expression is induced by proinflammatory cytokines in a variety of cell types, including in human adipocytes differentiated in vitro.Objective:Our objective was to determine the mechanisms by which proinflammatory cytokines induce 11β-HSD1 in human adipocytes.Results:The proinflammatory cytokines IL-1α (10 ng/mL) and TNFα (20 ng/mL) increased 11β-HSD1 mRNA levels in human primary adipocyte fractions and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes (P

U2 - 10.1210/jc.2013-1708

DO - 10.1210/jc.2013-1708

M3 - Article

C2 - 24243637

SN - 0021-972X

VL - 99

SP - E160-E168

JO - The Journal of Clinical Endocrinology & Metabolism (JCEM)

JF - The Journal of Clinical Endocrinology & Metabolism (JCEM)

IS - 1

ER -

Proinflammatory Cytokine Induction of 11Beta-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA

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CLINCIAL ACADEMIC TRAINING F/SHIP MR CHRISTOPHER WEST : DEVELOPMENT OF 3-D POLYMER SCAFFOLD THAT PROMOTES CHONDROGENESIS OF HUMAN PERIVASCULAR STEM CELLS

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Cristina Esteves

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