Properties of an adrenal medullary protein immuno-related to steroid acute regulatory (StAR) protein

YC Lo, SM MacKenzie, AF Howie, DK Apps, JI Mason, BC Williams, SD Morley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immune-reactive species to have a higher molecular weight (50 kDa) compared to StAR protein (30 kDa). Subcellular fractionation localised the 50 kDa species principally to the medulla cytosol. StAR peptide antiserum binding to both the 30 kDa and 50 kDa species could be specifically competed by the peptide antigen. These data suggest that the adrenal medullary immune-reactive species and StAR protein are distinct entities, which share some features in common.

Original languageEnglish
Pages (from-to)737-745
Number of pages9
JournalEndocrine research
Volume26
Issue number4
Publication statusPublished - 2000
Event9th Conference on the Adrenal Cortex - TORONTO, Canada
Duration: 17 Jun 200020 Jun 2000

Keywords

  • BOVINE

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