OBJECTIVE: To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age.
DESIGN: Prospective cohort study.
SETTING: Hospital infertility clinics and academic centers.
PATIENT(S): Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles.
MAIN OUTCOME MEASURE(S): AMH and antral follicle count (AFC) determined at a single visit on day 2-4 of the menstrual cycle.
RESULT(S): There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho -0.50 for AFC and -0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively.
CONCLUSION(S): The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.
- Antimullerian hormone
- antral follicle count
- ovarian follicle
- ovarian reserve
- reproductive life span
- ANTI-MULLERIAN HORMONE
- SPONTANEOUS MENSTRUAL-CYCLE
- MLLERIAN HORMONE