Projects per year
Abstract
The ubiquitin-proteasome system is vital to hormone-mediated developmental and stress responses in plants. Ubiquitin ligases target hormone-specific transcriptional activators (TAs) for degradation, but how TAs are processed by proteasomes remains unknown. We report that in Arabidopsis, the salicylic acid- and ethylene-responsive TAs, NPR1 and EIN3, are relayed from pathway-specific ubiquitin ligases to proteasome-associated HECT-type UPL3/4 ligases. Activity and stability of NPR1 were regulated by sequential action of three ubiquitin ligases, including UPL3/4, while proteasome processing of EIN3 required physical handover between ethylene-responsive SCFEBF2 and UPL3/4 ligases. Consequently, UPL3/4 controlled extensive hormone-induced developmental and stress-responsive transcriptional programs. Thus, our findings identify unknown ubiquitin ligase relays that terminate with proteasome-associated HECT-type ligases, which may be a universal mechanism for processive degradation of proteasome-targeted TAs and other substrates.
Original language | English |
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Article number | eabn4466 |
Journal | Science Advances |
Volume | 8 |
Issue number | 42 |
DOIs | |
Publication status | Published - 21 Oct 2022 |
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Dive into the research topics of 'Proteasome-associated ubiquitin ligase relays target plant hormone-specific transcriptional activators'. Together they form a unique fingerprint.Projects
- 3 Finished
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Cellular Functions of Proteasome-Associated Ubiquitin Ligase Activity
Spoel, S. (Principal Investigator) & Bayne, E. (Co-investigator)
1/12/19 → 1/04/23
Project: Research
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Understanding the influence of environmental signals on establishment of plant disease resistance
Spoel, S. (Principal Investigator)
16/03/18 → 15/03/20
Project: Research
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IMMUNE-EXPRESS: Proteasome-Mediated Gene Expression in Plant Immunity
Spoel, S. (Principal Investigator)
1/03/16 → 31/08/21
Project: Research